Alphalyse to present at EBF 2011

Alphalyse presents at the European Bioanalysis Forum (EBF) 2nd focus meeting on connecting strategies on analyzing large molecules with small molecular technologied

24 May 2011

Dr. Ejvind Mørtz speaks at the EBF meeting June 20-21, 2011, Brussels, with the talk “Sample Preparation for Protein Quantification by LC MS/MS”.

Conference topics include:

• New technology developments for the analysis of peptides and proteins
• Cutting edge analytical approaches
• Regulatory challenges and acceptance criteria
• Analytical strategies: LBA versus LC-MS/(MS)
• Biomarkers

http://bru2011.europeanbioanalysisforum.eu/

Abstract
The quantification of proteins, using isotope dilution LC-MS/MS and Multiple Reaction Monitoring (MRM) of specific peptides from the protein of interest, requires multiple complex sample preparation steps to obtain accurate and reproducible results.

The optimal sample preparation is protein and peptide specific and includes steps for protein denaturation, reduction/alkylation of disulfide bonds, enzymatic hydrolysis and peptide fractionation prior to the LC MS analysis. The complexity of the sample matrix has great influence on these steps, and is very different for PK/TK serum samples, protein manufacturing samples, cell lysis samples, milk samples, etc. The matrix may for example contain thousands of other proteins in serum samples, detergents and additives in manufacturing samples, or fat, phospholipids and fragments from cell lysis samples.

Alphalyse has as CRO gained experience from a variety of protein LC-MS quantification projects, some of which have been validated according to EMEA guidelines for clinical use.  Examples will be presented for quantification of protein vaccines, monoclonal antibodies, and therapeutic proteins, as well as troubleshooting and practical experiences gained from different projects.

For more information, see www.alphalyse.com/protein-quantification.html

Alphalyse is a specialized contract research organization (CRO) providing high-quality protein analysis service to support the research, manufacturing and clinical development of natural and recombinant proteins. The company provides through its PICK ‘n POST Protein Analysis services and Contract Analysis Services a range of unique high quality protein analysis to identify and characterize proteins. The services are used by the Biotech industry, Pharmaceutical companies and Academic Research Groups all over the world. Alphalyse has offices in Odense, Denmark, and Palo Alto, California.