face

Bioprocess optimization

HCP analysis based on SWATH mass spectrometry technology

Do you want to optimize your bioprocess for host cell protein profile?

Mass spectrometry is a great tool for optimizing your bioprocess. We identify and quantify individual host cell proteins in your process samples using top-of-the-line mass spectrometry technology no matter the complexity or purity of the sample.

You can also use our services to evaluate the clearance of individual and total HCP at different purification steps. To optimize your bioprocess; purification parameters can be compared to find the right conditions for an optimal HCP profile.

Our method is based on an in-solution digestion followed by LC MS/MS peptide analysis. We use the highly advanced Sciex TrippleTOF 6600 mass spectrometer using SWATH technology. We search the obtained MS data against an ion library of host cell proteins. The library will match your specific cell line and it includes your drug substance sequence.

The analysis includes:

  • Identification of HCPs in each process batch.
  • Comparison of HCPs in various process batches.
  • In-solution trypsin digestion and SWATH LC-MS/MS.
  • Complete list of HCPs with physiochemical properties (Mw, pI, AA sequence, database accession number).

Both the FDA and EMA are increasingly aware of mass spectrometry (MS) as an orthogonal method to ELISA. We can also help you identify HCPs in pre-clinical and clinical batches.

Services/Products
Protein analysis by Alphalyse

Identification of host cell proteins by in-solution digest & SWATH LC MS/MS

  • Identification of host cell proteins
  • SWATH LC-MS/MS analysis on Sciex TrippleTOF 6600 mass spectrometer
  • Database search in custom HCP Ion library
  • HCP list with pI, Mw, aa sequence, accesion number

$12,000.00

World Class Service
Quality analysis, presented in easy-to-understand reports
Info / Order
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Technical details

Sample requirements

Sample shipment

shipping option
Ship your protein samples by a courier company of your own choice

Place your samples into micro tubes and pack in a suitable transport box.

  • Contact a courier company of your choice (FedEx, UPS, DHL, TNT, World Courier etc.). Use the following sentence to describe the content: “Protein material – Non-toxic, non-hazardous, for research purpose only“.
  • Use cool packs or cooling units if your samples should be stored below 5°C/40°F
  • Ship the samples to the office closest to you (Canadian customers, please ship to Europe):
Customers in the USA
Alphalyse, Inc.
200 Page Mill Road, Suite 100
Palo Alto, CA, 94306
USA
Att: Jette Williams
Tel: (650) 543-3193
Customers outside of USA
Alphalyse A/S
Unsbjergvej 4 D
DK-5220, Odense
DENMARK
Att: Anette Rasmussen
Tel: +45 6310 6500
shipping option
Order a prepaid FedEx Air Waybill < 1 lb./ 500g

A prepaid FedEx Air Waybill can be used for fast and easy shipment of samples in packages up to 1 lb./ 500g.
We will generate a prepaid Air Waybill, prefilled with the correct information, and send it to you by e-mail (PDF-format) within 24 hours.
Then follow these simple instructions:

  • Place your samples into micro tubes. Pack in suitable transport box.
  • Print the PDF document and stick it on the transport box or just hand it to your FedEx officer upon pickup.
  • Call to request a pickup from your local FedEx office.

If you plan to send your samples stored at room temperature, this option is usually the right choice. If you fill your box with cooling packs, the weight will most often exceed 1 lb./500g.

shipping option
Order a prepaid FedEx Air Waybill < 10 lb./5kg (e.g. for packages with cooling packs)

This prepaid FedEx Air Waybill can be used for shipment of samples in packages up to 10 lb./ 5kg, with or without cooling packs.
We will generate a prepaid Air Waybill, prefilled with the correct information, and send it to you by e-mail (PDF-format) within 24 hours.
Then follow these simple instructions:

  • Place your samples into micro tubes. Pack them in a suitable transport box. Fill with cooling units/cold packs.
  • Print the PDF and stick it on the transport box or just hand it to your FedEx officer upon pickup.
  • Call to request a pickup from your local FedEx office.

If you plan to fill your transportation box with cooling packs, select this shipping option.

shipping option
Get a quote for shipment by World Courier

The World Courier service can be used for shipment of samples of all types. If you select this option, we will contact you to provide a quotation based on location and shipping conditions. You will receive a prepaid Air Waybill which will be invoiced together with your analysis results. This is usually a good option if FedEx does not service your location.
With the prepaid World Courier service you get:

  • GxP compliant processes to ensure maximal security and traceability
  • Door-to-door solution on a dedicated vehicle
  • Possibility of providing qualified packaging, temperature monitoring and dry ice
  • Shortest possible transit-time through “next flight out”-procedure
  • Customs clearance and help with paperwork

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