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1D and 2D PAGE protein separation

Determination of protein electrophoretic pattern by 1D PAGE and 2D PAGE

Do you need separation of proteins by 1D PAGE or 2d SDS PAGE?

We offer to separate the proteins of your drug sample. This is done by either 1D PAGE or 2D PAGE (polyacrylamide gel electrophoresis). We use Coomassie, silver or fluorescent staining for visualization of all proteins in the sample.

Do you follow the ICH Q6B? (Test Procedures and Acceptance Criteria for Biotechnological/Biological Products). The ICH Q6B guideline requires that you determine the electrophoretic pattern of your protein by 1D and 2D gel electrophoresis.

Our service includes:

  • 1D gel electrophoresis or 2d gel electrophoresis separation of your sample.
  • Coomassie, silver or fluorescence staining of the proteins.
  • For 1-D SDS PAGE gel we will load 1-30 ug of protein on gel.
  • For 2-D SDS PAGE we will load 30-300 ug of protein on gel.

You can order our 1D PAGE and 2D electrophoresis analysis in combination with many of our other services. For example you may wish to have a look at: • Protein Identification analysis.N-terminal Edman sequencing  • Identification of protein isoforms and protein degradation fragments. • Host Cell Protein analysis.

The order form and sample submission form is found further down on this page.

If you need more information before ordering, please send us request.

Services/Products
Protein analysis by Alphalyse

1D SDS PAGE with Coomassie staining of 3 concentrations

  • 1D PAGE separation of proteins
  • 1 run with 3 different loadings of 1, 10 and 20 ug
  • Mw 5-250 kDa approximately, NuPAGE 12% Bis-Tris gel
  • Coomassie staining

$1,600.00

World Class Service
Quality analysis, presented in easy-to-understand reports
Info / Order
Request quote
Protein analysis by Alphalyse

2D PAGE with Coomassie staining of proteins in drug sample

  • 2D PAGE separation of proteins, 2 gels loading 50 and 150 ug DS, IEF pH 3-10
  • Mw 10-120 kDa, NuPAGE 4-12% Bis-Tris gel
  • Coomassie staining

$2,300.00

World Class Service
Quality analysis, presented in easy-to-understand reports
Info / Order
Request quote
Protein analysis by Alphalyse

2D PAGE separation with fluoroscence or silver staining of proteins

  • 2D PAGE separation of proteins, 2 gels loading 25 and 50 ug protein, IEF pH 3-10
  • Mw 10-120 kDa, NuPAGE 4-12% Bis-Tris gel
  • Sensitive fluorescence or silver staining

$2,300.00

World Class Service
Quality analysis, presented in easy-to-understand reports
Info / Order
Request quote
Order analysis now
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