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Accurate molecular weight by LC-MS

Exact molecular weight of all proteins in your sample - accuracy of 10 ppm

Want to know the exact molecular weight of proteins in your sample?

Get exact molecular weight  of proteins up to 250 kDa, using top-of-the-line UV-LC ESI-MS with an accuracy of 10 ppm (+/- 1 Da for 100 kDa protein). We only need 10 μg of protein for detailed MW analysis.

Service includes:

  • Exact molecular weight of proteins in your sample
  • Determined by UV-LC ESI-MS
  • Accuracy 10 ppm
  • Comparison with theoretical molecular weight of drug substance

All peaks in the chromatogram are deconvoluted to provide exact masses of all proteins within each peak.

Applications:

The Mw of protein isoforms and correlation with the protein sequence enables detailed understanding of the protein heterogeneity.
The Mw of co-eluting proteins and integration of HPLC peak areas allows semi-quantitative analysis of protein contaminants and degradation fragments.

  • Detect protein oxidations and deamidation
  • Glycoprofile characterization
  • Identify contaminating proteins not well separated by UV-HPLC (coeluting proteins or shoulders etc.)
  • Stability study – characterize degradation products
  • Drug substance batch comparison

Send us a request so we can adjust the analysis to meet your specific needs

Services/Products
Protein analysis by Alphalyse

Mw of proteins in drug sample by standard UV-LC MS method

  • UV-LC trace and exact MW of proteins in sample
  • UV-LC ESI-MS, standard method, accuracy +/- 2.5 Da
  • Comparison with theoretical protein MW

$1,200.00

World Class Service
Quality analysis, presented in easy-to-understand reports
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Protein analysis by Alphalyse

Mw of proteins in drug sample by customized UV-LC MS method

  • UV-LC trace and exact MW of proteins above 1%
  • Customized UV-LC ESI-MS method; eg. column, gradient, MS conditions
  • Accuracy +/- 2.5 Da
  • Comparison with theortical MW of protein

World Class Service
Quality analysis, presented in easy-to-understand reports
Info / Order
Request quote

Electrospray Ionization Mass Spectrometry (ESI-MS)

In LC-ESI MS, the proteins and peptides are separated by reversed-phase HPLC coupled on-line to an electrospray mass spectrometer. In the ESI-MS proteins and peptides in liquid phase are sprayed through a capillary at high voltage into the MS instrument where the mass-over-charge ratio (m/z) is measured. For large biomolecules the electrospray process results in multiply protonated molecules with a distribution of ion species at m/z range from 1-3000. The mass of the molecule is determined using a deconvolution algorithm that calculates the mass of the intact non-protonated protein or peptide. The ESI process requires that the sample preparation is quite pure without interfering salts or detergents.

Sample Preparation Guidelines
Protein samples can be submitted in liquid or as lyophilized material.
The chromatographic protein purity should be >90%
Avoid detergents and keep buffer concentration at a minimum. LC-ESI MS  analysis can be done on samples containing small amounts of salts, urea or detergent, but the best result are obtained with low buffer strength in volatile solvents without detergents.
Minimum amount ~ 10-50 micrograms

Put the protein/peptide into microcentrifuge tube
Use Eppendorf Safe Lock tubes, or similar tubes from your lab
Lyophilize or speed vac the protein to a solid sample to ensure protein stability during shipment
Alternatively, freeze or refrigerate to +4°C for cold shipment of the liquid sample

Attention
Please note that MW determination of intact proteins by mass spectrometry CANNOT be conducted on samples from gels or PVDF membranes!

Ship your protein samples by a courier company of your own choice

Place your samples into micro tubes and pack in a suitable transport box.

  • Contact a courier company of your choice (FedEx, UPS, DHL, TNT, World Courier etc.). Use the following sentence to describe the content: “Protein material – Non-toxic, non-hazardous, for research purpose only“.
  • Use cool packs or cooling units if your samples should be stored below 5°C/40°F
  • Ship the samples to the office closest to you (Canadian customers, please ship to Europe):
Customers in the USA
Alphalyse, Inc.
200 Page Mill Road, Suite 100
Palo Alto, CA, 94306
USA
Att: Jette Williams
Tel: (650) 543-3193
Customers outside of USA
Alphalyse A/S
Unsbjergvej 4 D
DK-5220, Odense
DENMARK
Att: Anette Rasmussen
Tel: +45 6310 6500
Order a prepaid FedEx Air Waybill < 1 lb./ 500g

A prepaid FedEx Air Waybill can be used for fast and easy shipment of samples in packages up to 1 lb./ 500g.
We will generate a prepaid Air Waybill, prefilled with the correct information, and send it to you by e-mail (PDF-format) within 24 hours.
Then follow these simple instructions:

  • Place your samples into micro tubes. Pack in suitable transport box.
  • Print the PDF document and stick it on the transport box or just hand it to your FedEx officer upon pickup.
  • Call to request a pickup from your local FedEx office.

If you plan to send your samples stored at room temperature, this option is usually the right choice. If you fill your box with cooling packs, the weight will most often exceed 1 lb./500g.

Order a prepaid FedEx Air Waybill < 10 lb./5kg (e.g. for packages with cooling packs)

This prepaid FedEx Air Waybill can be used for shipment of samples in packages up to 10 lb./ 5kg, with or without cooling packs (do not use dry ice!).
We will generate a prepaid Air Waybill, prefilled with the correct information, and send it to you by e-mail (PDF-format) within 24 hours.
Then follow these simple instructions:

  • Place your samples into micro tubes. Pack them in a suitable transport box. Fill with cooling units/cold packs (do not use dry ice!).
  • Print the PDF and stick it on the transport box or just hand it to your FedEx officer upon pickup.
  • Call to request a pickup from your local FedEx office.

If you plan to fill your transportation box with cooling packs, select this shipping option. If you plan to use dry ice, you cannot use this Waybill!

Get a quote for shipment by World Courier

The World Courier service can be used for shipment of samples of all types. If you select this option, we will contact you to provide a quotation based on location and shipping conditions. You will receive a prepaid Air Waybill which will be invoiced together with your analysis results. This is usually a good option if FedEx does not service your location.
With the prepaid World Courier service you get:

  • GxP compliant processes to ensure maximal security and traceability
  • Door-to-door solution on a dedicated vehicle
  • Possibility of providing qualified packaging, temperature monitoring and dry ice
  • Shortest possible transit-time through “next flight out”-procedure
  • Customs clearance and help with paperwork

Order analysis now
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