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Accurate molecular weight by LC-MS

Exact molecular weight of all proteins in your sample - accuracy of 10 ppm

Want to know the exact molecular weight of proteins in your sample?

Get exact molecular weight  of proteins up to 250 kDa, using top-of-the-line UV-LC ESI-MS with an accuracy of 10 ppm (+/- 1 Da for 100 kDa protein). We only need 10 μg of protein for detailed MW analysis.

Service includes:

  • Exact molecular weight of proteins in your sample
  • Determined by UV-LC ESI-MS
  • Accuracy 10 ppm
  • Comparison with theoretical molecular weight of drug substance

All peaks in the chromatogram are deconvoluted to provide exact masses of all proteins within each peak.

Applications:

The Mw of protein isoforms and correlation with the protein sequence enables detailed understanding of the protein heterogeneity.
The Mw of co-eluting proteins and integration of HPLC peak areas allows semi-quantitative analysis of protein contaminants and degradation fragments.

  • Detect protein oxidations and deamidation
  • Glycoprofile characterization
  • Identify contaminating proteins not well separated by UV-HPLC (coeluting proteins or shoulders etc.)
  • Stability study – characterize degradation products
  • Drug substance batch comparison

Send us a request so we can adjust the analysis to meet your specific needs

Services/Products
Protein analysis by Alphalyse

Mw of proteins in drug sample by standard UV-LC MS method

  • UV-LC trace and exact MW of proteins in sample
  • UV-LC ESI-MS, standard method, accuracy +/- 2.5 Da
  • Comparison with theoretical protein MW

$1,200.00

World Class Service
Quality analysis, presented in easy-to-understand reports
Info / Order
Request quote
Protein analysis by Alphalyse

Mw of proteins in drug sample by customized UV-LC MS method

  • UV-LC trace and exact MW of proteins above 1%
  • Customized UV-LC ESI-MS method; eg. column, gradient, MS conditions
  • Accuracy +/- 2.5 Da
  • Comparison with theortical MW of protein

World Class Service
Quality analysis, presented in easy-to-understand reports
Info / Order
Request quote
Order analysis now
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