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N- and C-terminal sequencing

Top-down sequencing of intact proteins by MALDI-ISD

Do you want to confirm the N- and C-terminal sequence of your drug substance protein?

By using MALDI-ISD we can determine up to 80 amino acids from the N-terminal of your drug substance protein. Also, we map up to 80 amino acids from the C-terminal. N- and C-terminal sequencing by MALDI-ISD works for terminally modified proteins. Such as N-terminally blocked proteins, where traditional N-terminal Edman sequencing does not work. With MALDI-ISD truncated isoforms and post translationally modified terminals can be identified.

Do you follow the ICH Q6B? (Test Procedures and Acceptance Criteria for Biotechnological or Biological Products). The ICH Q6B requires that you provide an overview of the N- and C-term amino acid sequence of your drug substance. Our experts provide you with a report to use in your project to live up to the requirements.

Our service includes:

  • N- and C-terminal sequence of your sample analysis by MALDI-ISD.
  • Determination of 20-80 amino acids from each terminal of the protein.
  • Comparison with the expected drug substance sequence.
  • MALDI-MS intact protein Mw determination.

Below you will find our simple order form. Or you can send us a request so we can discuss the analyses for your specific needs.

Download application note (pdf – 766 kb)

Services/Products
Protein analysis by Alphalyse

N- and C-terminal sequencing drug substance – HPLC purification

  • N- & C-terminal sequence by MALDI-ISD
  • Standard UV-HPLC separation of sample
  • 1 fraction collected and analyzed, compared to DS sequence

$2,100.00

World Class Service
Quality analysis, presented in easy-to-understand reports
Info / Order
Request quote
Protein analysis by Alphalyse

N-terminal Edman sequencing of drug substance

  • N-terminal Edman sequencing, determination of 10 amino acids
  • Comparison with DS sequence
  • From pure protein in-solution, lyophilized, or PVDF gel blot

$900.00

World Class Service
Quality analysis, presented in easy-to-understand reports
Info / Order
Request quote
Protein analysis by Alphalyse

Peptide mapping looking for N- and C-terminal peptides

  • Digestion with 1-3 enzymes, e.g. trypsin, Lys-C
  • nanoLC-MS/MS, 60 min gradient
  • Common modifications included, e.g. deamidation, oxidation, truncations
  • Sequence coverage generally 75-85%

World Class Service
Quality analysis, presented in easy-to-understand reports
Info / Order
Request quote
Order analysis now
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