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N-terminal Edman degradation

Determination of N-terminal amino acid sequencing

Want to know the N-terminal amino acid sequence of your drug substance protein?

We can list up to 40 amino acids of your drug substance. To do this, we use N-terminal Edman degradation. Our experts perform N-terminal sequencing, using the newest technology.

Edman degradation is a cyclic procedure. We cleave off the amino acid residues one at a time. Then we identify them by chromatography. If the sample holds 2-3 sequence variants the degradation can show this. However, if the sample contains multiple N-terminals it is not possible to read the aa sequence.

Our N-terminal sequencing service includes:

  • Optional 1D SDS PAGE or HPLC isolation of the protein.
  • Sequencing by Edman degradation.
  • Determination of up to 40 amino acids.
  • Finally, a comparison to expected drug substance sequence.

We deliver the data in a report, which is easy to understand and use in your studies.

Download application note (pdf – 1 MB)

Not exactly what you are looking for? Then send us a request for a customized project proposal to meet your specific needs.

Need a faster and less expensive analysis? Take a look at our PICK ‘n POST Edman sequencing service. With this service you get the first 5, 10 or 20 amino acids analyzed in just 4-8 days!

Services/Products
Protein analysis by Alphalyse

N-terminal Edman sequencing of drug substance

  • N-terminal Edman sequencing, determination of 10 amino acids
  • Comparison with DS sequence
  • From pure protein in-solution, lyophilized, or PVDF gel blot

$900.00

World Class Service
Quality analysis, presented in easy-to-understand reports
Info / Order
Request quote
Protein analysis by Alphalyse

N-terminal Edman sequencing including 1D PAGE & PVDF blot

  • 1D PAGE separation, incl PVDF blot & staining
  • N-terminal Edman sequencing, determination of 10 amino acids
  • Comparison with protein sequence

$1,750.00

World Class Service
Quality analysis, presented in easy-to-understand reports
Info / Order
Request quote
Protein analysis by Alphalyse

N-terminal Edman sequencing of drug substance (customized)

  • Custom 1D PAGE, PVDF blot and band choice
  • Optional pGlu aminopeptidase deblocking
  • Edman sequencing, 10, 20 or more residues
  • Custom analysis for sequence variants, and semi-quantification

$2,000.00

World Class Service
Quality analysis, presented in easy-to-understand reports
Info / Order
Request quote
Order analysis now
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