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N-terminal Edman Sequencing

Sequencing 5-20 amino acids from protein N-terminal

The N-terminal Edman sequencing service provides detailed information about the N-terminal amino acid sequence of your protein. It is a cyclic procedure, where amino acid residues are cleaved off one at a time. This is followed by identification of each amino acid one-by-one by chromatography. The analysis can distinguish between isobaric amino acids like Ile/Leu.

Choose your service

Our PICK ‘n POST™ services are fast protein analysis without customization. You get quality results in easy to understand standard reports, at fixed prices.

5 amino acids

N-terminal Edman determination of 5 amino acids

  • PVDF membrane bound proteins
  • N-terminal Edman degradation, determination of 5 amino acids
  • Reporting include raw data
$450.00
Per sample
Results in 4-8 days
10 amino acids

N-terminal Edman determination of 10 amino acids

  • PVDF membrane bound proteins
  • N-terminal Edman degradation, determination of 10 amino acids
  • Reporting includes raw data
$650.00
Per sample
Results in 4-8 days
20 amino acids

N-terminal Edman determination of 20 amino acids

  • PVDF membrane bound proteins
  • N-terminal Edman degradation, determination of 20 amino acids
  • Reporting include raw data
$1,000.00
Per sample
Results in 4-8 days
Service includes
  • You get the first 5, 10 or 20 N-terminal amino acids.
  • We send the report to you within 4-8 days.

N-terminal HPLC chromatogram distingushing all 20 amino acids except Cysteine

Applications
Our customers typically use this analysis for the following applications:

Confirmation of N-terminal

Analysis of degradation product

Identification of enzymatic cleavage products

Sample requirements

This analysis is only performed on PVDF membrane bound proteins.
Use this guide for help on How to convert your sample to PVDF membrane bound proteins. You can also ask us to run the 1D/2D gels and transfer to PVDF membrane.

Protein sequencing analysis by N-terminal Edman degradation requires 2-10 micrograms, or 10-50 picomoles, of a pure protein. If a protein sample contains more than one protein, the analysis will show a mixture of amino acids in each Edman degradation cycle, and a sequence can not be determinated.

Technical details

The Edman degradation chemistry is a cyclic procedure where the PITC reagent is coupled to the free N-terminal amino group and cleaved off the protein. The PITC coupled residue is transferred to a flask, converted to a PTH-residue and the amino acid is identified by HPLC chromatography. The Edman degradation reaction cycle is repeated and a sequence of N-terminal amino acids is determined.

Since the Edman sequencing process never is 100% there will be a decrease in signal intensity as the process goes a long. Therefore the amount of material needed will increase with increased number of residues.

The Edman sequencing analysis will not work if the N-terminal amino group is blocked for the PITC chemistry due to e.g. acetylation or pyroglutamic acid which is frequent for antibodies. If the sample contains more than one protein, the chromatography will show multiple amino acids and a unique sequence cannot be read.

Have questions? We'd love to help.

We're always eager to help. Please send us your question or request and we will get back to you as soon as possible.

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