by Edman Degradation
Do you need to determine or verify the N-terminal amino acid sequence of a protein?
Cyclic Edman degradation is a well established method for confirmation of the N-terminus of proteins and peptides.
Typical applications of Edman sequencing are:
- Verification of the N-terminal sequence
- QC of recombinant proteins
- Quantification of N-terminal processing of leading Methionine
- Characterization of protein degradation products
The analysis is performed on in-solution proteins, or on SDS-PAGE separated proteins blotted onto a PVDF membrane. We can customize the analysis and correlate the raw data to your specific protein sequence and look for specific residues. Custom reports include, as part of our QC, a standard sample with 10 pmol beta-lactoglobulin and calculated repetitive yields, as well as the raw data for your sample.
You can read more in the application note: Applications of N-terminal Edman sequencing.
The N-terminal sequencing service is also offered as PICK 'n POST service for standard sequencing of 5, 10 or 20 residues.
Information about the N-terminus can also be obtained through our MALDI-ISD sequencing service.