N-terminal Sequencing

by Edman Degradation

Do you need to determine or verify the N-terminal amino acid sequence of a protein?

Cyclic Edman degradation is a well established method for confirmation of the N-terminus of proteins and peptides.

Typical applications of our sequencing service for:

• Verification of the N-terminal sequence
• QC of recombinant proteins
• Quantification of N-terminal processing of leading Methionine
• Characterization of protein degradation products

The analysis is performed on in-solution proteins, or on SDS-PAGE separated proteins blotted onto PVDF membranes. We can customize the analysis to your project and protein sequence. For example by correlating the raw data to the specific recombinant sequence and looking for modified residues. Custom reports include, as part of our QC, a standard sample with 10 pmol beta-lactoglobulin and calculated repetitive yields, as well as the raw data for your sample.

You can read more in the application note: Applications of N-terminal Edman sequencing.

The N-terminal sequencing service is also offered as PICK 'n POST service for standard sequencing of 5, 10 or 20 residues.

Information about the N-terminus can also be obtained through our MALDI-ISD sequencing service.