N-terminal Edman Sequencing
of proteins and peptides
Want to confirm & characterize the N-terminal amino acids of your protein?
The PICK 'n POST N-terminal Edman Sequencing service will provide the data within 2-8 days.
Automated Edman sequencing of the protein or peptide. The Edman degradation chemistry is a cyclic procedure where the PITC reagent is coupled to the N-terminal amino group and cleaved off the protein. The PITC coupled residue is transferred to a flask, converted to a PTH-residue and the amino acid is identified by HPLC chromatography. The Edman degradation reaction cycle is repeated and a sequence of N-terminal amino acids is determined.
You will receive
You will receive a detailed and easy to understand report (request sample report) by e-mail containing:
- Amino acid sequence interpreted from the analysis data
- Raw data HPLC chromatograms of each Edman degradation cycle