Antibody analysis

Structural characterization of antibodies and antibody-drug-conjugates

Alphalyse offers a range of services for structural characterization of monoclonal antibodies. Our services are suitable for monoclonal antibodies, antibody-drug-conjugates (ADCs) and also for antibody biosimilars. Thus you can used them to optimize the process development of your antibody product.

We always perform our tests according to the ICH Q6B guidelines. (Test Procedures and Acceptance Criteria for Biotechnological/Biological Products.)

Choose and combine your preferred analysis package from the following services:

Identification

We identify the antibody products in your sample by mass spectrometry.

Peptide mapping

We confirm the amino acid sequence of your mAbs by comparing to the theoretical sequence.

Sequencing

We determine the exact amino acid sequence of your mAbs. Either by N-terminal Edman or de-novo sequencing.

Molecular weight

We determine the exact molecular weight of intact and also reduced monoclonal antibodies.

Quantification

We quantify the precise amount of protein in your antibody product sample.

Modification

We determine the number and also position of disulfide bridges and glycosylations in your antibody.

HCP analysis

We identify and quantify host cell proteins in your product and provide physical properties of each HCP.

DAR profiling

We determine the exact distribution of drug-to-antibody ratio (DAR) and also the drug attachment site(s).

Antibody analysis

Choose and combine your preferred analysis package from the following services:

Send us your project description. We will help you design a service package that meets your needs.