Do you need the no. and position of disulfide bridges of mAb (monoclonal antibody)?
We offer to determine the number and position of disulfide bridges of mAb. We do this by disulfide mapping.
Disulfide analysis is an important part of characterizing recombinant monoclonal antibodies. The ICH Q6B guidelines require that you list the of number and position of disulfide bridges.
Our service includes:
Part 1: Determine the number of disulfide bridges and free sulfhydryl groups.
- We find the exact molecular weight of non-reduced mAb (intact bridges). This is determined by ESI-MS.
- We find the exact molecular weight of reduced mAb (broken bridges). This is determined by ESI-MS.
Part 2: Position of disulfide bridges of mAb determined by peptide mapping of non-reduced and reduced form.
- We digest any non-reduced mAb with 2-4 enzymes. Then we perform peptide mapping by LC-MS/MS.
- We digest reduced mAb with 2-4 enzymes. Afterwards, we perform peptide mapping by LC-MS/MS.
We use conditions to keep scrambling effects to a minimum.
Or send us a project request so we can design the analysis to meet your specific needs.