Quantification of free Amino Acids

Amino acid analysis service of free amino acids - without hydrolysis

Our scientists can help you with analysis of amino acids. Particularly those not bound up in a protein or peptide but exist freely in your sample. Thus we do not hydrolyze the sample prior to amino acid analysis. If your sample contains proteins, we include a purification step to remove proteins prior to the hplc analysis.


Choose your service

Our Fast Protein Analysis Services are without customization. You get quality results in easy to understand standard reports, at fixed prices.

Option 1

Quantification of free amino acids

  • Removal of proteins prior to analysis, no hydrolysis
  • Quantification of 18 standard amino acids
  • Accuracy within +/- 10%
Per sample
Results in 4-8 days
Service includes
  • First step is removal of proteins by precipitation prior to analysis.
  • Direct analysis without hydrolysis.
  • Next, we perform ion exchange chromatography on an amino acid analyzer.
  • Quantification of 20 amino acids with an accuracy of +/- 10%.
  • We deliver the report within 4-8 days.
HPLC chromatogram

HPLC chromatogram for free amino acid analysis on Biochrom instrument

Our customers typically use this analysis for the following applications:

Accurate determination of free amino acids for raw materials in food and consumer products

Optimization of fermentation processes and analysis of traces of amino acids

Measuring the changes in culture media amino acids over time

Sample requirements

Avoid high salt concentrations.
Buffer that contain high amounts of primary amines, e.g. TRIS or glycine, may interfere with the nihydrin reaction and quantification of specific amino acids

Samples can be provided as solid lyophilized powder or as liquid:

  • Provide 50-100 micro-liters (or more) of liquid material at a minimum concentration of 0.1 micro-gram per micro-liter or higher.
  • Solid material should be provided in sufficient amount for accurate weighing of samples at Alphalyse (mg material).

Unstable samples should be shipped refrigerate to +4°C as cold shipment.

Technical details

The normal 20 amino acids are detected and other unusual amino acids are also detected including hydroxyproline and hydroxylysine. The analysis includes

  • Sample preparation including removal of protein material
  • HPLC analysis
  • Data calculation

The analysis is performed on a BioChrom +30 amino acid analyser by ion-exchange chromatography with post-column derivatization using ninhydrin. All amino acids except Proline (440 nm) are monitored at 570 nm.

Have questions? We'd love to help.

We're always eager to help. Please send us your question or request and we will get back to you as soon as possible.

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