Host Cell Protein Analysis
Identification and quantitation of Host Cell Protein in biologics
Alphalyse probably offers the most detailed Host Cell Protein analysis available on the market. Where most other suppliers offer analysis based on ELISA kits or ELISA antibodies, we use advanced SWATH mass spectrometry to provide you with the total HCP content (ng HCP/mg DS) in each sample.
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Alphalyse probably offers the most detailed Host Cell Protein analysis available on the market. Where most other suppliers offer analysis based on ELISA kits or ELISA antibodies, we use advanced SWATH mass spectrometry to provide you with the total HCP content (ng HCP/mg DS) in each sample.
You also get the ID and quantity of each individual HCP. All of this in just 8-12 weeks – and without the need for a null-cell line.
Additionally, if you already use ELISA kits or antibodies, we can help you measure the HCP-antibody coverage. And if no commercial ELISA exists for your host cell line, mass spectrometry is the perfect solution for detecting and measuring host cell proteins, as well as other process-related impurities.
Applicable to all types of samples and expression systems
The approach can be applied to all types of process samples, from harvest to final drug product, and all types of expression systems, including E. coli, CHO, yeast, HEK, adenovirus, etc. The only requirement is that the host cell proteome must be sequenced and available – in public databases or in your possession.
Send us a request to start a dialogue on how the method can be applied to your samples and expression system.
Have a look at the various host cell protein analysis services below and contact us for more information – or attend one of our webinars:
Coverage analysis – Which HCPs is your ELISA missing? >>
Strategies for impurity analysis of Biologics >>
Impurity analysis for data-driven Process development >>
Impurity analysis using Mass Spec (method) >>