Host Cell Protein Analysis
Identification and quantitation of Host Cell Protein in biologics
Alphalyse probably offers the most detailed Host Cell Protein analysis available on the market. Where most other suppliers offer analysis based on ELISA kits or ELISA antibodies, we use advanced SWATH mass spectrometry.
How does ELISA compare to Mass Spectrometry (LC-MS)?
As with an ELISA, LC-MS provides ...
Alphalyse probably offers the most detailed Host Cell Protein analysis available on the market. Where most other suppliers offer analysis based on ELISA kits or ELISA antibodies, we use advanced SWATH mass spectrometry.
How does ELISA compare to Mass Spectrometry (LC-MS)?
As with an ELISA, LC-MS provides you with the total HCP content (ng HCP/mg DS) in each sample. But with LC-MS you also get the ID and quantity of each individual HCP. And it is fast – just 8-12 weeks – and without the need for a null-cell line.
Additionally, if you already use ELISA kits or antibodies, we can help you measure their HCP-antibody coverage.
If no commercial ELISA HCP assay exists for your host cell line, mass spectrometry is the solution for detecting and measuring host cell proteins, as well as other process-related impurities.
Applicable to all types of samples and expression systems
The approach can be applied to all types of process samples, from harvest to final drug product, and all types of expression systems, including E. coli, CHO, yeast, HEK, adenovirus, etc. The only requirement is that the host cell proteome must be sequenced and available – in public databases or in your possession.
Send us a request to start a dialogue on how the method can be applied to your samples and expression system.