HCP levels in clinical batches
- Compare the HCP levels between pre-clinical and clinical batches
- Orthogonal method to ELISA to document HCP content in clinical batches
- Regulatory documentation for FDA and EMA
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Set up meetingAnalyses of key protein features, stability and impurity levels
Explore customer caseInnovative development of an orthogonal method for analysis of Host Cell Proteins
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The SWATH mass spec technology is a data independent acquisition strategy used to measure hcp levels. This means that we collect all MS/MS fragmentation data for every peptide in your sample.
This is only possible due to the high speed of modern mass spectrometers.
The Sciex 6600 TripleTof instrument makes it possible to divide the whole mass range into sequential windows of size from 5-50 daltons. We can thus perform MS/MS fragmentation covering the full mass range (m/z 350-1700) within a few seconds. We set up the individual windows based on analysis of your samples, and typically get 50-100 windows.
In this way we obtain MS/MS data for all peptides without the instrument having to select certain peptides for MS/MS fragmentation.
The MS/MS data set links to the retention time and contains MS/MS data from multiple peptides. The extraction of these MS/MS data are done using a spectra library linking to peptide sequences in a library.
The spectra library can be prepared in many ways. The most simple way is to run a few samples in data dependent acquisition mode to collect good MS/MS data. Or it can be more advanced based on off-line fragmentation followed by MS/MS analysis of each fraction. The library can be extended with new MS/MS data at any given time, and used for a research of old SWATH data.
Analysis of HCP levels using SWATH technology requires sample material in sufficient amounts to obtain good data. It is important to prepare samples in a clean laboratory to avoid contamination with human keratin.
Submit samples in liquid or lyophilized.
Samples for Library generation and SWATH analysis