HCP profiling for bioprocess optimization
- Evaluate clearance of individual HCPs between purification steps
- Compare HCP profiles for tech-transfer batches between CMOs
- Monitor HCP content during process changes and scale-up
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Set up meetingInnovative development of an orthogonal method for analysis of Host Cell Proteins
Explore customer caseAnalyses of key protein features, stability and impurity levels
Explore customer caseWe would like to help you as much as possible with your project and therefore provide several kinds of customer support:
The SWATH, mass spec technology, is a data-independent acquisition strategy. It means that all MS/MS fragmentation data for every peptide in your sample is collected. However, it is only possible due to the high speed of modern mass spectrometers.
The Sciex 6600 TripleTof instrument makes it possible to divide the whole mass range into sequential windows of size from 5-50 daltons and still perform MS/MS fragmentation covering the entire mass range (m/z 350-1700) within a few seconds. We set up the separate windows based on the analysis of your samples and typically get 50-100 windows.
This way, we can obtain MS/MS data for all peptides without having the instrument to select specific peptides for MS/MS fragmentation.
The MS/MS data set is linked to the retention time and can contain MS/MS data from multiple peptides. These MS/MS data are extracted using a library’s spectra linking to peptide sequences.
The spectra library can be prepared in many ways. The simplest way is to run a few samples in data-dependent acquisition mode to collect good MS/MS data. Or it can be more advanced based on off-line fragmentation followed by MS/MS analysis of each fraction. Finally, the library can be extended with new MS/MS data at any given time and used to research old SWATH data.
HCP analysis using SWATH technology requires sample material in sufficient amounts to obtain good data. Also, samples must be prepared in a clean laboratory to avoid contamination with human keratin.
You may submit samples in liquid or lyophilized.