Quantification of Host Cell Proteins
- Determine HCPs specific to your process
- Obtain the exact amount in all purification steps
- Results to be used for removal of immunogenic and proteolytic proteins
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Set up meetingWe would like to help you as much as possible with your project and therefore provide several kinds of customer support:
The absolute quantification of specific protein in complex matrices are based on the selection of specific peptides as signature peptides. These are used for assay development based on Multiple-Reaction-Monitoring (MRM). The project are typically divided into three parts:
The purpose of the feasibility study is to investigate if we can develop an assay for quantification of the specific protein in the matrix. The feasibility study includes:
Protein quantification by mass spectrometry requires a protein in sufficient amounts to obtain good data. It is also important that samples are prepared in a clean laboratory to avoid contamination with human keratin. To develop MRM quantification assays requires the protein in pure form. Additionally, we need a matrix sample without the protein similar to the matrix of the real samples to be analyzed.
Protein samples can be submitted in liquid or lyophilized.