Quantification of Host Cell Proteins
- Determine HCPs specific to your process
- Obtain the exact amount in all purification steps
- Results to be used for removal of immunogenic and proteolytic proteins
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Set up meetingAnalyses of key protein features, stability and impurity levels
Explore customer caseDetailed host cell protein overview enables targeted elimination of impurities
Explore customer caseWe would like to help you as much as possible with your project and therefore provide several kinds of customer support:
The absolute quantification of a specific protein in complex matrices is based on selecting specific peptides as signature peptides. These are used for assay development based on Multiple-Reaction-Monitoring (MRM). The project is typically divided into three parts:
The feasibility study aims to investigate if we can develop an assay for quantifying the specific protein in the matrix. The feasibility study includes:
Protein quantification by mass spectrometry requires a protein in sufficient amounts to obtain good data. Developing MRM quantification assays requires the protein in pure form. Additionally, we need a matrix sample without the protein similar to the matrix of the actual samples to be analyzed. It is also important that samples are prepared in a clean laboratory to avoid contamination with human keratin.
Protein samples can be submitted in liquid or lyophilized.