Intact mass analysis or molecular weight determination (MW) of peptides, proteins and antibodies are a strong evidence for correct expression of specific proteins. It is also a fast analysis to verify the purity, analysis of post-translation modifications and eventual degradation products.
Top-of-the-line mass spectrometry instruments provide very accurate MW of both proteins and antibodies. We are able to perform the intact mass analysis on a few µg of protein. We determine the MW with an accuracy within 10 ppm (+/- 1 Da for a 100 kDa protein).
The intact mass analysis can be conducted before and after reduction, before and after de-glycosylation, and after specific digest forming sub-units.
You can choose between the following molecular weight determination services:
- Intact mass analysis of proteins or mAbs >
We determine the exact molecular weight of proteins or intact and reduced monoclonal antibodies in your sample.
- MW of ADCs for DAR profiling >
We determine the exact distribution of drug-to-antibody ratio (DAR) in your antibody-drug-conjugate.