Molecular weight determination

Exact intact mass of proteins or antibodies

Molecular weight determination of peptides, proteins and antibodies are strong evidence for correct expression of specific proteins. It is also a fast analysis to verify the purity, map a wide-range of post-translation modifications and eventual degradation products.

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Molecular weight determination of peptides, proteins and antibodies are strong evidence for correct expression of specific proteins. It is also a fast analysis to verify the purity, map a wide-range of post-translation modifications and eventual degradation products.

With our setup, you get access to top-of-the-line mass spectrometry instruments that provide very accurate molecular weight (MW) of both proteins and antibodies. We can perform the analysis on a few µg of protein. The analysis is very sensitive: In general, we can determine the weight with an accuracy within 10 ppm (+/- 1 Da for a 100 kDa protein).

Molecular weight determination can be conducted both before and after reduction. You can also use it before and after de-glycosylation, and even after specific digest forming sub-units.

Related services

This service is often an essential part of comprehensive protein characterization project. Together with peptide mapping, host cell protein analysis and amino acid analysis, it forms an overview of the protein stability, function, and purity.

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Our experts in protein analysis by mass spectrometry can help you in the following areas:

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What buffer should I use for MALDI mass spectrometry analysis of a protein?

In general, MALDI-MS is more tolerant to contamination than other MS methods. However, the salts and detergents that are commonly used in biomolecular science can still interfere. Either with crystallisation or ionisation or both.

What is the molecular weight of bovine serum albumin (BSA)?

In general, MALDI-MS is more tolerant to contamination than other MS methods. Questions like these are typical. Therefore, Alphalyse launched GPMAW lite protein molecular weight calculator to quickly make these calculations -> alphalyse.com/gpmaw-tool/

Who is this for?

We primarily help customers within the pharmaceutical industry, and biotechs working on drug development, e.g.

Project managers

Working with process development and characterization projects.

Analytical experts

Protein scientists that work with elisa, masss spectrometry, or CMC.

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