Molecular weight determination

Exact mass of proteins or antibodies

Molecular weight determination of peptides, proteins and antibodies are a strong evidence for correct expression of specific proteins. It is also a fast analysis to verify the purity, map a wide-range of post-translation modifications and eventual degradation products.

With o...

Molecular weight determination of peptides, proteins and antibodies are a strong evidence for correct expression of specific proteins. It is also a fast analysis to verify the purity, map a wide-range of post-translation modifications and eventual degradation products.

With our setup, you get access to top-of-the-line mass spectrometry instruments that provide very accurate molecular weight (MW) of both proteins and antibodies. We can perform the analysis on a few µg of protein. 

Molecular weight determination can be conducted both before and after reduction. You can also use it before and after de-glycosylation, and even after specific digest forming sub-units.

Services related to molecular weight determination

Molecular weight determination is often an essential part of comprehensive protein characterization project. When bundled with peptide mapping, host cell protein analysis and amino acid analysis, it forms an overview of the protein stability, function, and purity.

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Our experts in protein analysis by mass spectrometry can help you in the following areas:

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    What buffer should I use for mass spectrometry analysis of a protein?

    In general, most buffers work, but the concentration of the buffer components should not be too high. The salts and detergents that are commonly used in biomolecular science can still interfere and are of greater concern than the buffer. Either it can affect crystallisation or ionisation or both.

    What is the molecular weight of bovine serum albumin (BSA)?

    Questions like these are typical. Therefore, you can use the GPMAW lite protein molecular weight calculator to quickly make these calculations -> alphalyse.com/gpmaw-tool/

    Should molecular weight determination of protein be conducted before or after reduction?

    You can do both. And you can also use it before and after de-glycosylation.

    Who is this for?

    We primarily help customers within the pharmaceutical industry, and biotechs working on drug development, e.g.

    Project managers

    Working with process development and characterization projects.

    Analytical experts

    Protein scientists that work with ELISA, masss spectrometry, or CMC.

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