Molecular weight determination
Exact intact mass of proteins or antibodies
Molecular weight determination of peptides, proteins and antibodies are strong evidence for correct expression of specific proteins. It is also a fast analysis to verify the purity, map a wide-range of post-translation modifications and eventual degradation products.
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Molecular weight determination of peptides, proteins and antibodies are strong evidence for correct expression of specific proteins. It is also a fast analysis to verify the purity, map a wide-range of post-translation modifications and eventual degradation products.
With our setup, you get access to top-of-the-line mass spectrometry instruments that provide very accurate molecular weight (MW) of both proteins and antibodies. We can perform the analysis on a few µg of protein. The analysis is very sensitive: In general, we can determine the weight with an accuracy within 10 ppm (+/- 1 Da for a 100 kDa protein).
Molecular weight determination can be conducted both before and after reduction. You can also use it before and after de-glycosylation, and even after specific digest forming sub-units.
Related services
This service is often an essential part of comprehensive protein characterization project. Together with peptide mapping, host cell protein analysis and amino acid analysis, it forms an overview of the protein stability, function, and purity.