Molecular weight determination
Exact intact mass of proteins or antibodies
Molecular weight determination of peptides, proteins and antibodies are a strong evidence for correct expression of specific proteins. It is also a fast analysis to verify the purity, analysis of post-translation modifications and eventual degradation products.
Our top-of-the-line...
Molecular weight determination of peptides, proteins and antibodies are a strong evidence for correct expression of specific proteins. It is also a fast analysis to verify the purity, analysis of post-translation modifications and eventual degradation products.
Our top-of-the-line mass spectrometry instruments provide very accurate molecular weight of both proteins and antibodies. We can perform the analysis on a few µg of protein. The analysis is very sensitive: In general we can determine the weight with an accuracy within 10 ppm (+/- 1 Da for a 100 kDa protein).
Molecular weight determination can be conducted before and after reduction. You can also use it before and after de-glycosylation, and even after specific digest forming sub-units.
Determination of DAR distribution
In addition to our regular intact mass analysis we can also determine the exact distribution of drug-to-antibody ratio (DAR) in your ADC. Here we use the exact mass to determine the distribution of intact and de-glycosylated ADC and calculate the DAR distribution in percentage based on MS peak intensity.