Molecular weight determination

Exact intact mass of proteins or antibodies

Molecular weight determination of peptides, proteins and antibodies are a strong evidence for correct expression of specific proteins. It is also a fast analysis to verify the purity, analysis of post-translation modifications and eventual degradation products.

Our top-of-the-line...

Molecular weight determination of peptides, proteins and antibodies are a strong evidence for correct expression of specific proteins. It is also a fast analysis to verify the purity, analysis of post-translation modifications and eventual degradation products.

Our top-of-the-line mass spectrometry instruments provide very accurate molecular weight of both proteins and antibodies. We can perform the analysis on a few µg of protein. The analysis is very sensitive: In general we can determine the weight with an accuracy within 10 ppm (+/- 1 Da for a 100 kDa protein).

Molecular weight determination can be conducted before and after reduction. You can also use it before and after de-glycosylation, and even after specific digest forming sub-units.

Determination of DAR distribution

In addition to our regular intact mass analysis we can also determine the exact distribution of drug-to-antibody ratio (DAR) in your ADC. Here we use the exact mass to determine the distribution of intact and de-glycosylated ADC and calculate the DAR distribution in percentage based on MS peak intensity.

You can choose between the following  molecular weight determination services:

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What buffer should I use for MALDI mass spectrometry analysis of a protein?

In general, MALDI-MS is more tolerant to contamination than other MS methods. However, the salts and detergents that are commonly used in biomolecular science can still interfere. Either with crystallisation or ionisation or both.

What is the molecular weight of bovine serum albumin (BSA)?

In general, MALDI-MS is more tolerant to contamination than other MS methods. Questions like these are typical. Therefore, Alphalyse launched GPMAW lite protein molecular weight calculator to quickly make these calculations -> alphalyse.com/gpmaw-tool/

Who is this for?

We primarily help customers within the pharmaceutical industry, and biotechs working on drug development, e.g.

Project managers

Working with process development and characterization projects.

Analytical experts

Protein scientists that work with elisa, masss spectrometry, or CMC.

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