Do you need the exact intact mass of proteins or antibodies?
Intact mass analysis of proteins by ESI-MS and correlation with the sequences enables a detailed understanding of the protein heterogeneity. Also, you can use it to confirm correct protein expression.
Furthermore, the intact mass of co-eluting proteins/antibodies and integration of HPLC peak ar...
Do you need the exact intact mass of proteins or antibodies?
Intact mass analysis of proteins by ESI-MS and correlation with the sequences enables a detailed understanding of the protein heterogeneity. Also, you can use it to confirm correct protein expression.
Furthermore, the intact mass of co-eluting proteins/antibodies and integration of HPLC peak areas allow a semi-quantitative analysis of protein contaminants and degradation products.
When can you benefit from intact mass analysis?
- Detection of post-translational modifications, e.g., oxidations
- Glycoprofile characterization
- Identify contaminating proteins that are not well separated by UV-HPLC. (coeluting proteins or shoulders etc.)
- Stability study – characterize degradation products
- Drug substance batch comparison
PROTEIN MASS
Intact mass analysis by LC ESI-MS is the golden standard for proteins up to 250 kDa.
ANTIBODY MASS
It is possible to conduct the intact mass analysis with and without reduction. We also offer protein mass analysis before and after removing N-glycans and after enzymatic cleavage into specific sub-units.
Please send us a request so we can adjust the protein mass analysis to meet your specific needs.
