Novel +70 DA modification

31 August 2021

New article by scientists at Alphalyse on using mass spectrometry to measure a product-related impurity in a novel biologic:

N๐จ๐ฏ๐ž๐ฅโ€‰+โ€‰๐Ÿ•๐ŸŽ ๐ƒ๐š ๐ฆ๐จ๐๐ข๐Ÿ๐ข๐œ๐š๐ญ๐ข๐จ๐ง ๐ข๐ง ๐ซ๐ก๐†๐Œ-๐‚๐’๐… ๐ž๐ฑ๐ฉ๐ซ๐ž๐ฌ๐ฌ๐ž๐ ๐ข๐ง ๐„. ๐œ๐จ๐ฅ๐ข:

Authors: Magdalena Widgren Sandberg, Jakob Bunkenborg, Stine Thyssen, Martin Villadsen and Thomas Kofoed

The scientists found and identified a novel post-translational modification in fermentation batches of Granulocyte-macrophage colony-stimulating factor (GM-CSF) recombinantly expressed in E. coli.

The modification gave a mass shift ofโ€‰+โ€‰70 Da. Peptide mapping analysis demonstrated its location to the protein N-terminus and lysine side chains.

Full article (open access):

This study shows the importance of good analytics to detect and monitor product-related impurities during biologics development.