We just published a new blog post on the use of HILIC analysis of glycosylations.
In order to get a complete picture of your antibody’s glycosylations you need an analysis at different levels.
Typically, you conduct the following analyses:
- Top-down analysis of the intact mAb.
- Middle-up approach of the mAb subunits (LC, Fd´, and scFc).
- Bottom-up characterization of the N-glycopeptides.
- Free N-glycan analysis.
In the article Thomas Kofoed describes the different analysis possibilities. You also learn about the benefits of a HILIC analysis.
The HILIC solution is particularly beneficial for biologics. Biopharma companies thus use it for control of glycoprotein product quality and consistency.