Many biopharmaceutical proteins are cross-linked by disulfide bonds. They help increase the durability and protect from proteolytic degradation. The disulfide bridge pattern can therefore influence the efficacy and integrity of the final drug product. Thus disulfide bridge mapping is one of the services we often recommend for characterization of biologics.
In this short video you follow senior principal scientist Fen Yang Reske-Nielsen on a typical workday. She explains how she goes about a typical project. She also answers some common questions about the disulfide mapping procedure.
Watch video here: https://youtu.be/ev1RsJcxScI
Analysis typically include identification of the number and/or position of disulfide bridges present. We can also detect mismatched or scrambled disulfide bridge formation.
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