Peptide characterization
Detailed protein characterization by peptide map
Getting a peptide map is a critical part of any protein characterization project. You use it for confirming the amino acid sequence of your protein, and obtain detailed information about post translational modifications (PTMs). You can even locate truncations and cleavage sites.
The analysis is often applied as a vital part of comparability studies R...
Getting a peptide map is a critical part of any protein characterization project. You use it for confirming the amino acid sequence of your protein, and obtain detailed information about post translational modifications (PTMs). You can even locate truncations and cleavage sites.
The analysis is often applied as a vital part of comparability studies – especially in quality assurance/quality control (QA/QC) of protein-based biologics, such as mAbs, ADCs and also recombinant proteins.
High sequence coverage by specific proteases
The principle uses a specific protease that digests the protein and turns it into peptides. We then perform peptide characterization by LC-MS/MS using the peptide mass (MS) and the fragmentation pattern (MS/MS) for each peptide.
To get as high sequence coverage as possible it is important to select the right proteases. Therefore, we prefer to perform bioinformatics analysis of your specific protein to be able to advise you on the type and number of proteases to apply for your project. In this way, full sequence coverage is achieved, and you receive a detailed protein sequencing report.
We experience that many customers also combine peptide mapping with other services, such as N-terminal sequencing, de-novo sequencing and intact mass analysis, to extend the protein characterization.