Peptide characterization
Detailed protein characterization by peptide map
Getting a peptide map is a critical part of any protein characterization project. It is used to confirm the amino acid sequence of your protein, and obtain detailed information about post translational modifications (PTMs), as well as locate truncations and cleavage sites.
It is...
Getting a peptide map is a critical part of any protein characterization project. It is used to confirm the amino acid sequence of your protein, and obtain detailed information about post translational modifications (PTMs), as well as locate truncations and cleavage sites.
It is often applied as a vital part of comparability studies, especially in quality assurance/quality control (QA/QC) of protein-based biologics, such as mAbs, ADCs and recombinant proteins.
High sequence coverage by specific proteases
The principle is to use a specific protease for digestion turning the protein into peptides. We perform peptide characterization by LC-MS/MS using the peptide mass (MS) and the fragmentation pattern (MS/MS) for each peptide.
To get as high sequence coverage as possible it is important to select the right proteases. Therefore, we prefer to perform bioinformatics analysis of your specific protein to be able to advise you about the type and number of proteases to apply for your project. In this way, full sequence coverage is achieved, and you receive a detailed protein sequencing report.
The analysis is regularly combined with other services, such as N-terminal Edman sequencing, de-novo sequencing and intact mass analysis, to extend the protein characterization.