Peptide characterization

Detailed protein characterization by peptide map

Getting a peptide map is a critical part of any protein characterization project. You use it to confirm the amino acid sequence of your protein, and obtain details about post translational modifications (PTMs). Peptide analysis can even locate truncations and cleavage sites.

The analysis is often a vital part of comparability studies – especially ...

Getting a peptide map is a critical part of any protein characterization project. You use it to confirm the amino acid sequence of your protein, and obtain details about post translational modifications (PTMs). Peptide analysis can even locate truncations and cleavage sites.

The analysis is often a vital part of comparability studies – especially in quality assurance/quality control (QA/QC) of protein-based biologics. These include mAbs, ADCs and also recombinant proteins.

High sequence coverage by specific proteases

The principle uses a specific protease that digests the protein and turns it into peptides. We then perform peptide characterization by LC-MS/MS. More specifically, we use the peptide mass (MS) and the fragmentation pattern (MS/MS) for each peptide.

To get the highest sequence coverage possible it is important to select the right proteases. Therefore, we first perform bioinformatics analysis of your specific protein to advise on type and number of proteases to apply for your project. This way, full sequence coverage is achieved, and you receive a detailed protein sequencing report.

Experience shows that many customers also combine peptide mapping with other services. Therefore, w also offer N-terminal sequencing, de-novo sequencing and intact mass analysis, to extend the protein characterization.

You may select your preferred peptide characterization analysis from below services:

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    Is it possible to compare batch-to-batch variations in N-glycosylations?

    The HILIC workflow provides a reproducible, accurate, sensitive and fast glycan assessment. Therefore, it is very useful for routinely monitoring of critical glycoforms.

    What could be the cause of decreased protein stability?

    Since disulfide bonds provide stability, problems with the protein’s stability and activity often correlate with an abnormal disulfide bridge pattern. For many proteins and peptides, disulfide bridges are even essential for optimal biologic function.

    How do I characterize the N-glycosylation of an antibody?

    For a comprehensive characterization of N-glycosylation of a monoclonal antibody (mAb) you would typically use four complementary HILIC MS-based analysis techniques for top-down analysis, middle-up approach, bottom-up characterization and free N-glycan analysis.

    What type of peptide analysis is most common?

    A peptide map can confirm the amino acid sequence and give detailed information about post translational modifications - and even locate truncations and cleavage sites

    Who is this for?

    We primarily help customers within the pharmaceutical industry, and biotechs working on drug development, e.g.

    Project managers

    Working with process development and characterization projects.

    Analytical experts

    Protein scientists that work with ELISA, masss spectrometry, or CMC.

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