Peptide characterization
Detailed sequence analysis by quantitative peptide mapping
Getting a peptide map is a critical part of any analysis project. You use it to confirm the amino acid sequence of your protein and quantify post-translational modifications (PTMs). Peptide analysis can locate truncations and cleavage sites and identify degradation products with stabilit...
Getting a peptide map is a critical part of any analysis project. You use it to confirm the amino acid sequence of your protein and quantify post-translational modifications (PTMs). Peptide analysis can locate truncations and cleavage sites and identify degradation products with stability studies.
The analysis is often vital in comparability studies, especially in quality assurance/quality control (QA/QC) of protein-based biologics. These include mAbs, ADCs, and also recombinant proteins.
How to get high sequence coverage with specific proteases
The principle uses a particular protease that digests the protein and turns it into peptides. We then perform peptide characterization by LC-MS/MS. More specifically, we use the peptide mass (MS) and the fragmentation pattern (MS/MS) for each peptide.
To get the highest sequence coverage possible, it is important to select the right proteases. Therefore, the first step is to perform bioinformatics analysis of your specific protein to advise on the type and number of proteases to apply to your project. This way, full sequence coverage is achieved, and you receive a detailed report.
Experience shows that many customers also combine peptide mapping with other services. Therefore, you may want to add intact mass analysis to extend the protein characterization.