Peptide mapping is a critical part of any protein characterization project. It is used to confirm the amino acid sequence of your protein, and also to obtain detailed information about post translational modifications (PTMs) and eventual truncations.
The principle is to use a specific protease for digestion turning the protein into peptides. We perform peptide characterization by LC-MS/MS using the peptide mass (MS) and the fragmentation pattern (MS/MS) for each peptide.
To get as high sequence coverage as possible it is important to select the right proteases. We will therefore like to perform bioinformatics analysis of your specific protein to be able to advise you about the type and number of proteases to apply for your project.
You can choose your preferred analysis from the following services:
- Confirmation of protein, mAb or ADC sequence >
We confirm the theoretical amino acid sequence of your drug substance by MALDI MS/MS, nano LC-MS/MS or UV LC-MS/MS.
- Disulfide bridge analysis of protein or mAb >
We determine both the number and position of all disulfide bridges in your drug substance protein or monoclonal antibody.
- Glycosylation analysis of protein or mAb >
We determine both the number and position of all glycosylations in your drug substance protein or monoclonal antibody.