Modification analysis (PTM)

Characterization of post translational modifications

Do you suspect or observe issues with your biopharmaceutical product’s stability?
– It might be related to the protein’s post translational modifications (PTMs), which are important for e.g. cell signaling, function and stability.

Or are you in need of the analytical capabilities to investigate the number and position of different modificati...

Do you suspect or observe issues with your biopharmaceutical product’s stability?
– It might be related to the protein’s post translational modifications (PTMs), which are important for e.g. cell signaling, function and stability.

Or are you in need of the analytical capabilities to investigate the number and position of different modifications?
– As you might know, the characterization of these may be quite challenging, to say the least.

Alphalyse can help you with a range of analytical services for determination of PTMs, including a highly detailed and comprehensive analysis of disulfide bridges and glycosylations. You can find more information about these analyses on our blog and in the links below.

Customized search for specific post translational modifications

However, we can also look at e.g. deamidation, oxidation, methylation and citrullination with our peptide mapping service. Essentially, we can search for all modification types in the Delta Mass Database of PTMs using Mascot software. This is not a part of the standard service but send us a request and we will prepare a customized project proposal for you.

The post translational modifications are typically investigated using a combination of protein chemistry and mass spectrometry. Thus, the analytical design of these experiments requires deep understanding of the technology. This includes protein chemistry methods, advanced mass spectrometry instrumentation and software for data interpretation.

We hold large expertise in this area. Feel free to contact us for advice on your project. You can also receive help with other areas of protein characterization, such as peptide mapping.

You can choose between the following PTM analysis services:

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Is it possible to compare batch-to-batch variations in N-glycosylations?

The HILIC workflow provides a reproducible, accurate, sensitive and fast glycan assessment. Therefore, it is very useful for routinely monitoring of critical glycoforms.

What could be the cause of decreased protein stability?

Since disulfide bonds provide stability, problems with the protein’s stability and activity often correlate with an abnormal bridge pattern. For many proteins and peptides, disulfide bridges are even essential for optimal biologic function.

How do I characterize the N-glycosylation of my antibody?

For a comprehensive characterization of N-glycosylation of a monoclonal antibody (mAb) you would typically use four complementary HILIC MS-based analysis techniques for top-down analysis, middle-up approach, bottom-up characterization and free N-glycan analysis.

Who is this for?

We primarily help customers within the pharmaceutical industry, and biotechs working on drug development, e.g.

Project managers

Working with process development and characterization projects.

Analytical experts

Protein scientists that work with ELISA, masss spectrometry, or CMC.