Modifications

Characterization of post translational modifications

Most biologics contain post-translational modifications (PTMs) and the characterization of these can be quite challenging.

Alphalyse offers a range of analytical services for determination of PTMs, including analysis of disulfide bridges and gly...

Most biologics contain post-translational modifications (PTMs) and the characterization of these can be quite challenging.

Alphalyse offers a range of analytical services for determination of PTMs, including analysis of disulfide bridges and glycosylations.

The modifications are typically investigated using a combination of protein chemistry and mass spectrometry. Thus, the analytical design of these experiments requires deep understanding of the technology. This includes protein chemistry methods, advanced mass spectrometry instrumentation and software for data interpretation.

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Is it possible to compare batch-to-batch variations in N-glycosylations?

The HILIC workflow provides a reproducible, accurate, sensitive and fast glycan assessment. Therefore, it is very useful for routinely monitoring of critical glycoforms.

What could be the cause of decreased protein stability?

Since disulfide bonds provide stability, problems with the protein’s stability and activity often correlate with an abnormal disulfide bridge pattern. For many proteins and peptides, disulfide bridges are even essential for optimal biologic function.

How do I characterize the N-glycosylation of my antibody?

For a comprehensive characterization of N-glycosylation of a monoclonal antibody (mAb) you would typically use four complementary HILIC MS-based analysis techniques for top-down analysis, middle-up approach, bottom-up characterization and free N-glycan analysis.

Who is this for?

We primarily help customers within the pharmaceutical industry, and biotechs working on drug development, e.g.