Modification analysis (PTM)

Characterization of post translational modifications

Do you suspect or observe issues with your biopharmaceutical product’s stability? Analysis will show if it is related to a post translational modification in your protein (PTMs). It is important because it may influence e.g. cell signaling, function and stability.

Or, perhaps you need analytical capabilities to investigate the number and position of dif...

Do you suspect or observe issues with your biopharmaceutical product’s stability? Analysis will show if it is related to a post translational modification in your protein (PTMs). It is important because it may influence e.g. cell signaling, function and stability.

Or, perhaps you need analytical capabilities to investigate the number and position of different modifications?

Characterization of these may be quite challenging, to say the least. Alphalyse can help you with a range of analytical services for determination of PTMs. For instance a highly detailed and comprehensive analysis of disulfide bridges and glycosylations. You can find more information about these analyses on our blog and in the links below.

Would you like to search for specific post translational modifications?

However, we can also look at e.g. deamidation, oxidation, methylation and citrullination with our peptide mapping service. Essentially, we can search for all modification types in the Delta Mass Database of PTMs using Mascot software. This is not a part of the standard service but send us a request and we will prepare a customized project proposal for you.

The post translational modifications are typically investigated using a combination of protein chemistry and mass spectrometry. Thus, the analytical design of these experiments requires deep understanding of the technology. This includes protein chemistry methods, advanced mass spectrometry instrumentation, and also software for data interpretation.

We hold a large expertise in this area. Feel free to contact us for advice on your project. You can also receive help with other areas of protein characterization, such as peptide mapping.

You can choose between the following PTM analysis services:

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How do I compare batch-to-batch variations in glycosylations?

The HILIC workflow provides reproducible and accurate glycan analsyis and is useful for routinely monitoring of critical glycoforms.

What causes decreased protein stability?

Problems with the protein’s stability and activity often correlate with an abnormal bridge pattern. For many proteins and peptides, disulfide bridges are even essential for optimal biologic function.

Common forms of post translational modifications

Modifications of protein include abnormal bridge pattern, glycosylations, deamidation, oxidation, methylation, citrullination

Who is this for?

We primarily help customers within the pharmaceutical industry and biotechs working on drug development, e.g.

Project managers

Working with process development and characterization projects.

Analytical experts

Protein scientists that work with ELISA, masss spectrometry, or CMC.

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