You send peptide in-solution. The sample is analyzed by LC-MS/MS (30 minute gradient). The MS data is searched against public peptide sequence databases or custom designed databases.
The peptide or peptide mixture is analyzed by high resolution LC-MS/MS analysis. The analysis is perform on reverse phase chromatography coupled directly to high resolution mass spectrometer. The MS-MS spectra are used for Mascot database searching. The data are searched against in-house protein database downloaded from UniProt containing all known non-redundant protein sequences or custom designed databases.
Database search
The Mascot software finds matching peptides in the database by their peptide masses and peptide fragment masses. The peptide identification is based on a probability-scoring algorithm (www.matrixscience.com) and the significant best matching peptide is shown in the Results.
Reporting
The identified database peptide sequences are shown in the Results together with the obtained mass spectrometric peptide maps. The peptides used for the identification are highlighted in the sequence and the matching peptides are listed for comparison of the determined and calculated values.
Sample preparation
In-solution samples
Peptides samples can be submitted in solution or lyophilized. Amount ~ 5-50 micrograms
Avoid detergents, DMSO, glycerol and other non-volatile solvents. Keep buffer concentration at a minimum as high salt will interfere with the ionization in the MS. LC-ESI MS can be done on samples containing small amounts of salts, urea or detergent, but the best result are obtained with low buffer strength in volatile solvents without detergents.
Use a Eppendorf Safe Lock tubes, or similar tubes from your lab
Lyophilize or speed vac the peptide to a solid sample to ensure peptide stability during shipment
Alternatively, freeze the sample or refrigerate to +4 oC for cold shipment of the liquid sample.
Place your samples into micro tubes and pack in a suitable transport box. Remember to print and include the sample submission form.
| Customers in the USA Alphalyse, Inc. 299 California Avenue, Ste 200 Palo Alto, CA 94306 USA Att: Thomas Kofoed Tel: (650) 543-3193 | Customers outside of USA Alphalyse A/S Roedegaardsvej 209 C DK-5230 Odense M DENMARK Att: Anette Rasmussen Tel: +45 6310 6500 |
A prepaid FedEx Air Waybill can be used for fast and easy shipment of samples in packages up to 1 lb./ 500g.
We will generate a prepaid Air Waybill, prefilled with the correct information, and send it to you by e-mail (PDF-format) within 24 hours.
Then follow these simple instructions:
If you plan to send your samples stored at room temperature, this option is usually the right choice. If you fill your box with cooling packs, the weight will most often exceed 1 lb./500g.
This prepaid FedEx Air Waybill can be used for shipment of samples in packages up to 10 lb./ 5kg, with or without cooling packs (do not use dry ice!).
We will generate a prepaid Air Waybill, prefilled with the correct information, and send it to you by e-mail (PDF-format) within 24 hours.
Then follow these simple instructions:
If you plan to fill your transportation box with cooling packs, select this shipping option. If you plan to use dry ice, you cannot use this Waybill!
The World Courier service can be used for shipment of samples of all types. If you select this option, we will contact you to provide a quotation based on location and shipping conditions. You will receive a prepaid Air Waybill which will be invoiced together with your analysis results. This is usually a good option if FedEx does not service your location.
With the prepaid World Courier service you get:
My name is Anette and I am customer support scientist at Alphalyse. I make sure we take good care of your samples. If you need help, e.g. ordering or shipping samples, send me a message - I'm here to help.
