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  • ID of peptide – LC-MS/MS (in-solution)
Home / Standard analysis / Identification / ID of peptide – LC-MS/MS (in-solution)
Technical details
Sample preparation
Sample shipment

Identification of single or multiple proteins
Product number: 17858

ID of peptide – LC-MS/MS (in-solution)

$1,000.00
per sample

You send peptide in-solution. The sample is analyzed by LC-MS/MS (30 minute gradient). The MS data is searched against public peptide sequence databases or custom designed databases.

$1,000.00

Per sample



Total:

Fast and Easy delivery
Fast and Easy
Will be delivered in 4-8 days

  • LC-MS/MS peptide analysis
  • Search against peptide sequence database
  • ID of 1-100 peptides
Quantity:
Input can not be left blank
$1,000.00
per sample
$
per sample
Fast delivery
Results in 10-14 days
  • Number of samples

  • Detailed sample information

    Enter information about your samples for improved analysis quality.
  • Sample NameSample TypeConcentrationProtein MWAmountOrganismType of staining, buffer composition, expected purity 
  • Comments

  • If you have further comments to your order, you can write them here

Select database: * 

Simple search in Swiss-Prot is included. Confirmation of specific sequence or search in the complete UniProtKB or custom database can be added at an extra cost (per order).

Search Uniprot

If you are unsure whether your target protein and organism is found within Swiss-Prot or requires an expanded search in the complete UniProtKB database, we recommend to look it up on UniProt.

Technical details
Get more info
Sample preparation
Follow these directions
Sample shipment
Online guide

The peptide or peptide mixture is analyzed by high resolution LC-MS/MS analysis. The analysis is perform on reverse phase chromatography coupled directly to high resolution mass spectrometer.  The MS-MS spectra are used for Mascot database searching. The data are searched against in-house protein database downloaded from UniProt containing all known non-redundant protein sequences or custom designed databases.

Database search
The Mascot software finds matching peptides in the database by their peptide masses and peptide fragment masses. The peptide identification is based on a probability-scoring algorithm (www.matrixscience.com) and the significant best matching peptide is shown in the Results.

Reporting
The identified database peptide sequences are shown in the Results together with the obtained mass spectrometric peptide maps. The peptides used for the identification are highlighted in the sequence and the matching peptides are listed for comparison of the determined and calculated values.

Sample preparation

In-solution samples

Peptides samples can be submitted in solution or lyophilized. Amount ~ 5-50 micrograms

Avoid detergents, DMSO, glycerol and other non-volatile solvents. Keep buffer concentration at a minimum as high salt will interfere with the ionization in the MS. LC-ESI MS can be done on samples containing small amounts of salts, urea or detergent, but the best result are obtained with low buffer strength in volatile solvents without detergents.

Use a Eppendorf Safe Lock tubes, or similar tubes from your lab

Lyophilize or speed vac the peptide to a solid sample to ensure peptide stability during shipment

Alternatively, freeze the sample or refrigerate to +4 oC for cold shipment of the liquid sample.

Alphalyse shipping
Ship your protein samples by a courier company of your own choice

Place your samples into microtubes and pack them in a suitable transport box. Remember to print and include the sample submission form.

  • Contact a courier company of your choice (FedEx, UPS, DHL, TNT, World Courier, etc.). Use the following sentence to describe the content: “Protein material – Non-toxic, non-hazardous, for research purpose only.”
  • For storage below 5°C/40°F, use cool packs or cooling units.
  • Ship the samples to the office closest to you (Canadian customers, please ship to Europe):
Customers in the USA
Alphalyse, Inc.
299 California Avenue, Ste 200
Palo Alto, CA 94306
USA
Att: Thomas Kofoed
Tel: (650) 543-3193
Customers outside of the USA
Alphalyse A/S
Roedegaardsvej 209 C
DK-5230 Odense M
DENMARK
Att: Anette Rasmussen
Tel: +45 6310 6500

 

Alphalyse shipping
Order a prepaid FedEx Air Waybill < 1 lb./ 500g

You can use our prepaid FedEx Air Waybill to quickly ship samples in packages up to one lb./ 500g.

We will generate a prepaid Air Waybill, prefilled with the correct information, and send it to you by e-mail (PDF format) within 24 hours.

Then follow these simple instructions:

  • Place your samples into microtubes. Pack in a suitable transport box. Remember to print and include the sample submission form.
  • Print the PDF document and stick it on the transport box or hand it to your FedEx officer upon pickup.
  • Call to request a pickup from your local FedEx office.

If you plan to send your samples stored at room temperature, this option is usually the right choice. If you fill your box with cooling packs, the weight will often exceed one lb./500g.

Alphalyse shipping
Order a prepaid FedEx Air Waybill < 10 lb./5kg (e.g. for packages with cooling packs)

Select this shipping option if you plan to fill your transportation box with cooling packs. If you plan to use dry ice, you cannot use this Waybill!

The prepaid FedEx Air Waybill can be used to ship samples in packages up to 10 lb./ 5kg, with or without cooling packs (do not use dry ice!).

We will generate a prepaid Air Waybill, prefilled with the correct information, and send it to you by e-mail (PDF format) within 24 hours.

Then follow these simple instructions:

  • Place your samples into microtubes. Pack them in a suitable transport box. Remember to print and include the sample submission form.
  • Fill with cooling units/cold packs (do not use dry ice!).
  • Print the PDF and stick it on the transport box or hand it to your FedEx officer upon pickup.
  • Call to request a pickup from your local FedEx office.
Alphalyse shipping
Get a quote for shipment by World Courier

You can use the World Courier service to ship samples of all types.

If you select this option, we will contact you to provide a quotation based on location and shipping conditions. You will receive a prepaid Air Waybill, which will be invoiced together with your analysis results.

World Courier service is usually a good option if FedEx does not service your location.

With the prepaid World Courier service, you get:

  • GxP compliant processes to ensure maximal security and traceability
  • Door-to-door solution on a dedicated vehicle
  • Possibility of providing qualified packaging, temperature monitoring, and dry ice
  • Shortest possible transit-time through the “next flight out” procedure
  • Customs clearance and help with paperwork
If, for any reason, you cannot use our online order system, please fill in this sample submission form instead, and ship it with your samples.

I'm here to help you!

My name is Anette and I am customer support scientist at Alphalyse. I make sure we take good care of your samples. If you need help, e.g. ordering or shipping samples, send me a message - I'm here to help.

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Europe

Alphalyse A/S
Roedegaardsvej 209 C
Odense M, 5230 Denmark
(+45) 6310 6500

CVR register no: 26753864
VAT-ID: DK26753864


USA

Alphalyse, Inc.
299 California Av. St 200
Palo Alto, CA, 94306 USA
(650) 543-3193

TIN: 26-2109559

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