Protein Identification

Identify your proteins and peptides by mass spectrometry

Are you looking for a fast and reliable service for identification of the proteins /peptides in your solution or the bands in your gel?

With a comparison of the peptide map to public or custom databases?

Along with the physiochemical properties and link to the database entry you also receive the obtained sequence coverage maps. Here, the peptides...

Are you looking for a fast and reliable service for identification of the proteins /peptides in your solution or the bands in your gel?

With a comparison of the peptide map to public or custom databases?

Along with the physiochemical properties and link to the database entry you also receive the obtained sequence coverage maps. Here, the peptides, used for identification, are highlighted in the sequence.

Do you need to identify a PROTEIN?

Our protein Identification service using mass spectrometry is a very powerful and versatile tool. Especially since you can use it to identify or verify a single protein. However, you can also identify multiple proteins in complex samples. The analysis works equally well for both in-gel samples or in-solution samples. The service consists of a digest of protein material using a protease, followed by MS and then MS/MS sequencing.

The protein identification is made by matching the obtained data to the reviewed public protein database from Swiss-Prot.

Are you looking for a PEPTIDE?

You can also get peptide identification by MS and MS/MS sequencing. However, this analysis only works for in-solution samples. To get an accurate identification of the peptides in your solution, we search the acquired MS data against the SwissProt or UniProtKB databases.

Do you need high-throughput protein identification service of many samples? Or do you need to search against a custom database?

Then please send us a project proposal request. We do our best to get back to you within 24 hours.

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    Which electrophoresis gels can I use for MS protein identification?

    Polyacrylamide gels work well. Protein separation can be one-dimensional, like SDS-PAGE where proteins are separated by mass. Or, two dimensional, like 2D-PAGE where isoelectric point separates the proteins in the first dimension and mass in the second.

    What gel staining methods work for protein identification analysis?

    Use any type of Coomassie blue non-covalent dye for protein identification analysis. Fluorescent dyes like Sypro Ruby and Deep Purple are also compatible.

    How much material is needed to identify protein?

    You obtain good protein identification analysis data with 50 femtomole or more loaded onto gel.

    How do I avoid contaminating samples with Keratin?

    Minimize keratin contamination by e.g. wearing lab coat and non-latex gloves. Additionally, keep everything touching the gel free of dust, e.g. by washing with 70% ethanol.

    Who is this for?

    We primarily help customers within the pharmaceutical industry, and biotechs working on drug development, e.g.

    Project managers

    Working with process development and characterization projects.

    Analytical experts

    Protein scientists that work with ELISA, masss spectrometry, or CMC.

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