face

Identification

Identify your proteins and peptides by mass spectrometry analysis

PROTEIN

Protein identification using mass spectrometry is a very powerful tool and very versatile. First of all you can use it identify or verify a single protein. However, you can also identify multiple proteins in complex samples. The analysis works equally well for both in-gel samples or in-solution samples. The service consists of a digest of protein material using a protease, followed by MS and then MS/MS sequencing.

The protein identification is made by matching the obtained data to the public protein database from UniProt. This is the best database available, as it contains more than 100 million known protein sequences from thousands of organisms.

Multiple protein ID  >
First, we perform an in-gel or in-solution trypsin digest, followed by a peptide analysis by nanoLC-MS/MS. Afterwards, we search your data against a protein sequence database, with more than 100 million protein sequences. Thus, we can make an very accurate protein identification.

Single protein ID  >
We start with an in-gel trypsin digest, which is followed by peptide analysis by MALDI MS/MS. Then we search your data against a sequence database, with more than 100 million protein amino acid sequences available.

PEPTIDE

It is also possible to perform a peptide identification by MS and MS/MS sequencing. Our analysis works only for in-solution samples. To get an accurate identification of the peptides in your solution, we search the acquired MS data against the UniProt database.

Peptide ID  >
We perform LC-MS analysis of your in-solution samples. Thereby we identify either a single peptide or multiple peptides in the complex solution.

Do you need high-throughput protein/peptide identification of many samples? Or do you need to search against a custom database? – Then please send us a project proposal request. We will get back to you within 24 hours.


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