NanoLC-MS/MS analysis is the preferred method for identifying proteins – in either a simple or complex mixture. The sample format can be in-solution, in-gel bands, or spots from SDS gel electrophoresis.
The service includes digestion using a highly specific pro...
NanoLC-MS/MS analysis is the preferred method for identifying proteins – in either a simple or complex mixture. The sample format can be in-solution, in-gel bands, or spots from SDS gel electrophoresis.
The service includes digestion using a highly specific protease to generate peptides from the protein(s). We separate peptides using nano-flow HPLC coupled to a High-Resolution mass spectrometer to achieve the highest sensitivity. As a result, the method identifies thousands of peptides by their mass and fragmentation pattern.
We then search the data against the reviewed public Swiss-Prot database. To ensure access to the newest data, we update the databases monthly.
The nanoLC-MS/MS service includes:
- You provide us with a gel band containing one or multiple proteins.
- We perform in-gel trypsin digest and then nanoLC-MS/MS peptide analysis.
- We then search the data against the protein sequence database for protein identity.
- ID of typically up to 10-40 proteins per sample.
- Finally, you receive the report within 10-14 days.
Please send us a description of your project if you need a tailored project proposal.