Peptide identification

  • Identification within a few days
  • High accuracy with top-of-the-line instruments
  • For single purified peptides or peptide mixtures

Do you need peptide identification analysis of a complex mixture?

LC-MS/MS analysis is perfect for identifying and detailed characterization of a single or multiple peptides in a complex solution.

We separate the peptides using reverse-phase HPLC coupled to a high-resolution mass spectrometer, thus ensuring the highest sensitivity. Afterward, our scientists detect and identify the peptides from their mass and fragmentation...

Do you need peptide identification analysis of a complex mixture?

LC-MS/MS analysis is perfect for identifying and detailed characterization of a single or multiple peptides in a complex solution.

We separate the peptides using reverse-phase HPLC coupled to a high-resolution mass spectrometer, thus ensuring the highest sensitivity. Afterward, our scientists detect and identify the peptides from their mass and fragmentation pattern by searching the data against peptide databases. Typically, they apply UniProt or a custom-designed peptide database.

Standard peptide identification service:

  • The first step is the identification using LC-MS/MS.
  • Next follows a search of data against the sequence database for peptide identity.
  • Finally, we prepare a report – which you receive within a few days.

Please use the contact form below if you need a tailored project proposal. Alternatively, visit this page for information about more complex peptide characterization

MoreLess details

The process:

  • 1 You provide us with your sample
  • 2 Analysis with LC-MS/MS and data search against relevant databases
  • 3 Identification of 1-100 peptide(s) per sample
  • 4 You receive the report within 10-14 days

Learn more:

Let's work together

We offer customized solutions, contact us to discuss your project.

Ask a question or get a quote

Send us a description of your project and we shall be happy to present you with a solution.

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Knowledge center

More information

We would like to help you as much as possible with your project and therefore provide several kinds of customer support:

Technical details

Peptide or peptide mixture analysis by high-resolution LC-MS/MS

We perform the analysis using reverse phase chromatography coupled directly to a high-resolution mass spectrometer. Next, we use the MS-MS spectra for Mascot database searching. Typically, we search the data against in-house protein databases downloaded from UniProt. The Uniprot database thus contains all known non-redundant protein sequences or custom-designed databases.

Database search

The Mascot software finds matching peptides in the database by their peptide masses and peptide fragment masses. It bases peptide identification on a probability-scoring algorithm (www.matrixscience.com). Thus, the results show the significant best matching peptide.

Reporting

The report contains the identified database peptide sequences and the mass spectrometric peptide maps. The sequence highlights the peptide (s) used for the identification. A list of the matching peptides makes it easy to compare the determined and calculated values.

Sample preparation

Submit peptide samples for identification in-solution or lyophilized

Lyophilize or speed vac the peptide to a solid sample to increase peptide stability during shipment.

Alternatively, freeze the sample or refrigerate it to +4 oC for cold shipment of the liquid sample.

Minimum sample amount: 5-50 micrograms

Avoid detergents, DMSO, glycerol, and other non-volatile solvents. Keep buffer concentration at a minimum because high salt amounts interfere with the ionization in the MS. LC-ESI MS is sometimes possible for samples containing small amounts of salts or urea. However, we obtain the best results with low buffer strength in volatile solvents without detergents.

Use Eppendorf Safe-Lock tubes or similar high-quality tubes from your lab.

 

OBS. If your sample contains any detergent, you should send us a request through the contact form to inquire about the analytical capabilities of your project.

Meet the experts

For this type of analysis our experts include:

Do you need help?
Line Kofoed

PhD in Health and Medical Sciences

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Do you need help?
Li Peng Lundgren

PhD in Protein Chemistry

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