Peptide characterization

  • Identification within days
  • High accuracy with top-of-the-line instruments
  • For single purified peptides or peptide mixtures

Do you need peptide identification analysis of a complex mixture?

LC-MS/MS analysis is perfect for detailed characterization of a single peptide or multiple peptides in a complex solution.

To achieve the highest sensitivity, peptides are separated using reverse phase HPLC coupled to a high resolution mass spectrometer. As a result, peptides can be detect...

Do you need peptide identification analysis of a complex mixture?

LC-MS/MS analysis is perfect for detailed characterization of a single peptide or multiple peptides in a complex solution.

To achieve the highest sensitivity, peptides are separated using reverse phase HPLC coupled to a high resolution mass spectrometer. As a result, peptides can be detected and identified by its mass and fragmentation pattern searching data against peptide databases. The applied database is typically UniProt or custom designed peptide databases.

The standard service includes:

  • We perform LC-MS/MS peptide identification
  • We search the data against sequence database for peptide identity
  • Finally, you receive the report within few days

Please send us a request with a description of your project, so we can tailor your project proposal. 

MoreLess details

The process:

  • 1 You provide us with your sample
  • 2 Analysis with LC-MS/MS and data search against relevant databases
  • 3 We determine 1-100 peptides per sample
  • 4 You receive the report within 4-8 work days

More information:

See an example of how results are presented

Get sample report Peptide characterization analysis

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Knowledge center

More information

We would like to help you as much as possible with your project and therefore provide several kinds of customer support:

Technical details

The peptide or peptide mixture is analyzed by high resolution LC-MS/MS analysis

The analysis is performed on reverse phase chromatography coupled directly to high resolution mass spectrometer. The MS-MS spectra are used for Mascot database searching. The data are searched against in-house protein databases downloaded from UniProt containing all known non-redundant protein sequences or custom designed databases.

Database search

The Mascot software finds matching peptides in the database by their peptide masses and peptide fragment masses. The peptide identification is based on a probability-scoring algorithm (www.matrixscience.com) and the significant best matching peptide is shown in the Results.

Reporting

The identified database peptide sequences are shown in the Results together with the obtained mass spectrometric peptide maps. The peptides used for the identification are highlighted in the sequence and the matching peptides are listed for comparison of the determined and calculated values.

Sample preparation

Peptide samples can be submitted in-solution or lyophilized

Lyophilize or speed vac the peptide to a solid sample to increase peptide stability during shipment.

Alternatively, freeze the sample or refrigerate to +4 oC for cold shipment of the liquid sample.

Minimum sample amount: 5-50 micrograms

Avoid detergents, DMSO, glycerol and other non-volatile solvents. Keep buffer concentration at a minimum as high salt will interfere with the ionization in the MS. LC-ESI MS can be done on samples containing small amounts of salts or urea, but the best results are obtained with low buffer strength in volatile solvents without detergents.

Use Eppendorf Safe Lock tubes or similar high-quality tubes from your lab.

 

OBS. If your sample contains any type of detergent, send us a request through the contact form to hear about the analytical capabilities for your project.

Meet the experts

For this type of analysis our experts include:

Do you need help?
Anette Rasmussen

BSc in Biomedicine

Read more
Do you need help?
Per Aaslo

MSc in Molecular Biology & Environmental Biology

Read more
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