Protein separation
Separation of proteins from sample mixture
HPLC and SDS PAGE are two of the most common protein separation techniques. They are thus used to distinguish proteins and peptides by their physiochemical properties.
Alphalyse can help you with both protein separation techniques. For instance, we can set up any customer method within our ...
HPLC and SDS PAGE are two of the most common protein separation techniques. They are thus used to distinguish proteins and peptides by their physiochemical properties.
Alphalyse can help you with both protein separation techniques. For instance, we can set up any customer method within our lab and optimize the HPLC methods for specific purposes.
Protein separation can stand alone and provide you with information about specific proteins. However, you can also combine with other analyses to purify the sample beforehand. For instance when a HPLC system is coupled to a mass spectrometer.
When to use UV-HPLC:
It can also be helpful to combine the HPLC analysis with mass spectrometry. Then you can to obtain additional information about chromatographic peaks and stained spots on gels. Our protein separation services rely on more than 15 years of experience working with these analyses.
Separation by SDS PAGE gel bands:
Protein separation in SDS gel bands is often a vital part of analyses such as protein ID by mass spectrometry. If you prefer staining and cutting gels for protein identification yourself, we recommend that you check our protocols. Please send a request for combinations of protein separation and protein analysis by mass spectrometry.
You can read more about the protein separation services below.
You may also want to combine with our other analyses: Protein identification, peptide identification, process-related impurity analysis or post-translational modifications.