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Protein analysis by HPLC

  • Characterization of unknown peaks and shoulders
  • Stability study
  • Custom method setup
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Technical details, sample info, experts etc.

Determine the liquid chromatographic pattern of protein in drug sample

Our UV HPLC analysis service can play an important part in you protein characterization:

A chromatography may leave you with questions about the identity of specific peaks and shoulders. However, a molecular weight analysis of the peaks ...

Determine the liquid chromatographic pattern of protein in drug sample

Our UV HPLC analysis service can play an important part in you protein characterization:

A chromatography may leave you with questions about the identity of specific peaks and shoulders. However, a molecular weight analysis of the peaks by mass spectrometry will often identify the molecule.

Thus, our HPLC analysis service team can help you with:

  • Standard or customized HPLC analysis of your sample.
  • Collection of fractions for further analysis.
  • Combination with ESI-MS analysis for molecular weight determination of each peak, N-terminal Edman Sequencing and peptide mapping analysis.

Do you need to set up a custom HPLC analysis method?

You can also ask us to customize a method that meets your specific needs. For instance you may have an in-house method, which you want us to set up in our laboratory. If needed, we can also combine the service with high resolution Mw determination by mass spectrometry.

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The process:

  • 1 Contact us to discuss your project and receive a project proposal
  • 2 Analysis phase, where a project leader is appointed to manage the project
  • 3 You receive the report within a few weeks, depending on the analysis
  • 4 Follow-up by phone or email

Learn more:

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We offer customized solutions, contact us to discuss your project.

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Testimonials

  • "They quickly presented an optimized method that provided excellent data and clearly separated product related impurities."

    Optimized HPLC analysis of peptides for clinical trials and stable GMP production

    See more

  • "We are very pleased Alphalyses high-quality antibody characterization services. We ship hundreds of samples and always receive the analytic results shortly after."

    High-throughput characterization analysis of antibodies for fast quality assessment

    See more

Knowledge center

More information

We would like to help you as much as possible with your project and therefore provide several kinds of customer support:

Technical details

Introduction to UV HPLC analysis

The protein analysis is conducted on our Agilent 1290 HPLC systems equipped with fraction collector, UV and fluorescence detector.

Standard analysis

We have set of standard analysis including column and gradient settings.

Custom designed analysis

The custom designed analysis is based on HPLC settings from clients. It thus includes e.g. column, temperature, and also gradient settings. The analysis can be coupled to an MS instrument to obtain accurate mass information of all peaks.

HPLC analysis service - chromatogram with protein mixture

UV-HPLC Chromatogram of a protein mixture

Sample preparation

Sample requirements for UV HPLC analysis

Protein samples can be submitted in liquid form or as lyophilized material.

Minimum amount of material ~ 20-50 micrograms.

Avoid detergents and keep the buffer concentration at a minimum. HPLC data analysis may work on samples containing small amounts of salts or urea. However, the best results are obtained with low buffer strength in volatile solvents without detergents.

  • Put the sample into microcentrifuge tube.
  • Use Eppendorf Safe Lock tubes, or similar tubes from your lab.
  • Then lyophilize or speed vac the sample to a solid to ensure protein stability during shipment.
  • Alternatively, you can freeze or refrigerate to +4°C for cold shipment of the liquid sample.
Related services

You can combine this analysis with other analyses such as:

Meet the experts

For this type of analysis our experts include:

Do you need help?
Martin Villadsen

MSc in Engineering within Medical Biotechnology

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Do you need help?
Li Peng

PhD in Protein Chemistry

Read more
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