Protein analysis by HPLC
- Characterization of unknown peaks and shoulders
- Stability study
- Custom method setup
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Technical details, sample info, experts etc.
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- Protein analysis by HPLC
The process:
- 1 Contact us to discuss your project and receive a project proposal
- 2 Analysis phase, where a project leader is appointed to manage the project
- 3 You receive the report within a few weeks, depending on the analysis
- 4 Follow-up by phone or email
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We offer customized solutions, contact us to discuss your project.
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Send us a description of your project and we shall be happy to present you with a solution.
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Set up meetingTestimonials
"They quickly presented an optimized method that provided excellent data and clearly separated product related impurities."
Optimized HPLC analysis of peptides for clinical trials and stable GMP production
Explore customer case"We are very pleased Alphalyses high-quality antibody characterization services. We ship hundreds of samples and always receive the analytic results shortly after."
High-throughput characterization analysis of antibodies for fast quality assessment
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Knowledge center
More information
We would like to help you as much as possible with your project and therefore provide several kinds of customer support:
Technical details
Introduction to UV HPLC analysis
The protein analysis is conducted on our Agilent 1290 HPLC systems equipped with fraction collector, UV and fluorescence detector.
Standard analysis
We have set of standard analysis including column and gradient settings.
Custom designed analysis
The custom designed analysis is based on HPLC settings from clients. It thus includes e.g. column, temperature, and also gradient settings. The analysis can be coupled to an MS instrument to obtain accurate mass information of all peaks.
UV-HPLC Chromatogram of a protein mixture
Sample preparation
Sample requirements for UV HPLC analysis
Protein samples can be submitted in liquid form or as lyophilized material.
Minimum amount of material ~ 20-50 micrograms.
Avoid detergents and keep the buffer concentration at a minimum. HPLC data analysis may work on samples containing small amounts of salts or urea. However, the best results are obtained with low buffer strength in volatile solvents without detergents.
- Put the sample into microcentrifuge tube.
- Use Eppendorf Safe Lock tubes, or similar tubes from your lab.
- Then lyophilize or speed vac the sample to a solid to ensure protein stability during shipment.
- Alternatively, you can freeze or refrigerate to +4°C for cold shipment of the liquid sample.
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