Protein Sequencing

Determine amino acid sequence of protein, peptide and mAb

Do you want to identify or confirm the amino acid (AA) sequence of your protein, antibody, or peptide? Do you need a service to pinpoint truncations or cleavage sites? Or even quantify post-translational modifications?

Amino acid sequencing of proteins, antibodies, and peptides is...

Do you want to identify or confirm the amino acid (AA) sequence of your protein, antibody, or peptide? Do you need a service to pinpoint truncations or cleavage sites? Or even quantify post-translational modifications?

Amino acid sequencing of proteins, antibodies, and peptides is important in detailed sequence analysis and protein characterization. But amino acids sequencing approaches must be robust, reproducible, and sensitive to obtain full sequence coverage. Therefore, the protein sequencing service provided by Alphalyse uses liquid chromatography methods coupled with high-resolution mass spectrometry technology.

Comprehensive characterization package

In the past, the Edman degradation analysis was often used for N-terminal sequencing. But today, applying a combination of intact mass analysis and peptide mapping to obtain N- and C-terminal sequence information is more common. Thus, we mainly use MS technology for quantitative peptide maps to search specific protein databases. But we can also make peptide maps without protein sequence information.

You often combine protein sequencing with HPLC methods, such as RP, SEC, and IEX, and host cell protein analysis to characterize the biopharmaceutical product.

Send us a request if you would like us to prepare a bespoke project package. For instance, to get a complete overview of the biologic’s stability, function, and impurity profile.

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Our services

Our experts in protein analysis by mass spectrometry can help you in the following areas:

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We offer customized solutions, contact us to discuss your project.

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What is Sequence Coverage?

This is the number of amino acids in a protein sequence found in the peptides observed by mass spectrometry

Who is this for?

We primarily help customers within the pharmaceutical industry and biotechs working on drug development, e.g.

Project managers

Working with process development projects.

Analytical experts

Protein scientists that work with ELISA, masss spectrometry, or CMC.

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