Protein Sequencing
Determine amino acid sequence of protein, peptide and mAb
Do you want to identify or confirm the amino acid (AA) sequence of your protein, antibody, or peptide? Do you need a service to pinpoint truncations or cleavage sites? Or even quantify post translational modifications?
Amino acid sequencing of proteins, antibodies, and peptides pl...
Do you want to identify or confirm the amino acid (AA) sequence of your protein, antibody, or peptide? Do you need a service to pinpoint truncations or cleavage sites? Or even quantify post translational modifications?
Amino acid sequencing of proteins, antibodies, and peptides play an important part of detailed sequence analysis and protein characterization. But sequencing approaches must be robust, reproducible and sensitive to obtain full sequence coverage. Therefore, the protein sequencing service provided by Alphalyse is based on the original Edman degradtion method – and high-resolution mass spectrometry technology.
Should you use Edman protein sequencing or mass spectrometry?
The Edman degradation analysis provides up to 40 amino acid residues from the N-terminal – without any prior knowledge about the protein sequence. For quantitative peptide maps we mainly use MS technology to search specific protein databases. But we can also make peptide maps without protein sequence information.
We often combine protein sequencing with intact mass analysis, host cell protein analysis, etc. to fully characterize the biopharmaceutical product.
Send us a request if you would like us to prepare a custom project package. For instance to get the complete overview of the biologic’s stability, function, and impurity profile.