Protein Sequencing
Determine amino acid sequence of protein, peptide and mAb
Do you want to identify or confirm the amino acid (AA) sequence of your protein, antibody, or peptide? To pinpoint truncations or cleavage sites? Or even establish the sequence de-novo?
Amino acid sequencing of proteins, antibodies, and peptides plays an i...
Do you want to identify or confirm the amino acid (AA) sequence of your protein, antibody, or peptide? To pinpoint truncations or cleavage sites? Or even establish the sequence de-novo?
Amino acid sequencing of proteins, antibodies, and peptides plays an important part of detailed sequence analysis and protein characterization. But sequencing approaches must be robust, reproducible and sensitive to obtain full sequence coverage. Therefore, the protein sequencing service provided by Alphalyse is based on the original Edman degradtion method – and high-resolution mass spectrometry technology.
By Edman protein sequencing or mass spectrometry
The Edman degradation analysis provides up to 40 AA residues from the N-terminal – without any prior knowledge about the protein sequence. The MS technology is mainly used as part of peptide mapping to search specific protein databases but can also be performed without protein sequence information (de-novo sequencing).
We often combine sequencing of protein with intact mass analysis, analysis of post translational modifications, host cell protein analysis, etc. to fully characterize the biopharmaceutical product.
Send us a request if you would like us to prepare a custom project package that provides you with the complete overview of the biologic’s stability, function, and impurity profile.
You can combine your preferred protein sequencing package from the following services: