Protein Sequencing

Determine amino acid sequence of protein, peptide and mAb

Do you want to identify or confirm the amino acid (AA) sequence of your protein, antibody, or peptide? Do you need a service to pinpoint truncations or cleavage sites? Or perhaps even establish the sequence de-novo?

Amino acid sequencing of proteins, antib...

Do you want to identify or confirm the amino acid (AA) sequence of your protein, antibody, or peptide? Do you need a service to pinpoint truncations or cleavage sites? Or perhaps even establish the sequence de-novo?

Amino acid sequencing of proteins, antibodies, and peptides play an important part of detailed sequence analysis and protein characterization. But sequencing approaches must be robust, reproducible and sensitive to obtain full sequence coverage. Therefore, the protein sequencing service provided by Alphalyse is based on the original Edman degradtion method – and high-resolution mass spectrometry technology.

Should you use Edman protein sequencing or mass spectrometry?

The Edman degradation analysis provides up to 40 amino acid residues from the N-terminal – without any prior knowledge about the protein sequence. For peptide mapping we mainly use MS technology to search specific protein databases. But we can also perform peptide mapping without protein sequence information (de-novo sequencing).

We often combine protein sequencing with intact mass analysis, analysis of post translational modifications, host cell protein analysis, etc. to fully characterize the biopharmaceutical product.

Send us a request if you would like us to prepare a custom project package. For instance to get the complete overview of the biologic’s stability, function, and impurity profile.

You can combine your own protein sequencing service package below:

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    Typical questions

    What is de-novo protein sequencing?

    De-novo sequencing of monoclonal antibodies combines tandem MS (nanoLC-MS/MS) with database search. The method is especially useful if the parent hybridoma cell is not available for DNA sequencing. But is also used when the amino acid sequence does not match the DNA sequence. You may also add Edman sequencing to obtain the heavy- and light chains.

    What is 'Sequence Coverage'?

    This is the number of amino acids in a protein sequence found in the peptides observed by mass spectrometry.

    Can I identify a protein from an organism where the genome has not been sequenced?

    With de-novo peptide sequencing you can obtain the full protein sequencing - but you cannot identify the protein if it is completely novel.

    Who is this for?

    We primarily help customers within the pharmaceutical industry, and biotechs working on drug development, e.g.

    Project managers

    Working with process development and characterization projects.

    Analytical experts

    Protein scientists that work with ELISA, masss spectrometry, or CMC.

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