Determination of amino acid sequence of protein, peptide and antibody
Amino acid sequencing (AA) of proteins, antibodies, and peptides is an important part of detailed sequence analysis. Therefore, the protein sequencing service provided by Alphalyse is based on the original Edman degradation and high-resolution mass spectrometry technology.
The N-terminal Edman degradation analysis provides up to 40 AA residues from the N-terminal without any prior knowledge about the protein sequence. The MS technology is mainly used as part of peptide mapping to search specific protein databases but can also be performed without sequence information (de-novo sequencing).
You can combine your preferred package from the following protein sequencing services:
- N-terminal Edman degradation >
We determine up to 40 amino acids from the N-terminal of your protein or peptide. - Peptide mapping against sequence >
We compare the sequence of your protein to the theoretical sequence by trypsin digest and subsequently MALDI-MS or LC-MS/MS analysis. - N- and C-terminal sequencing >
We determine of up to 80 N- and C-terminal amino acids in your drug substance protein by MALDI-ISD (Top-down sequencing). - De-novo sequencing of antibody >
We determine the exact amino acid sequence of your monoclonal antibodies by de-novo.