Protein Sequencing

Determine amino acid sequence of protein, peptide and mAb

Do you want to identify or confirm the amino acid (AA) sequence of your protein, antibody, or peptide? Do you need a service to pinpoint truncations or cleavage sites? Or even establish the sequence de-novo?

Amino acid sequencing of proteins, antibodies, a...

Do you want to identify or confirm the amino acid (AA) sequence of your protein, antibody, or peptide? Do you need a service to pinpoint truncations or cleavage sites? Or even establish the sequence de-novo?

Amino acid sequencing of proteins, antibodies, and peptides plays an important part of detailed sequence analysis and protein characterization. But sequencing approaches must be robust, reproducible and sensitive to obtain full sequence coverage. Therefore, the protein sequencing service provided by Alphalyse is based on the original Edman degradtion method – and high-resolution mass spectrometry technology.

By Edman protein sequencing or mass spectrometry

The Edman degradation analysis provides up to 40 AA residues from the N-terminal – without any prior knowledge about the protein sequence. The MS technology is mainly used as part of peptide mapping to search specific protein databases but can also be performed without protein sequence information (de-novo sequencing).

We often combine protein sequencing with intact mass analysis, analysis of post translational modifications, host cell protein analysis, etc. to fully characterize the biopharmaceutical product.

Send us a request if you would like us to prepare a custom project package that provides you with the complete overview of the biologic’s stability, function, and impurity profile.

You can combine your own protein sequencing service package below:

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    What is de-novo protein sequencing?

    De-novo sequencing of monoclonal antibodies combines tandem MS (nanoLC-MS/MS) and database searching with PEAKS and Mascot software. The method is especially useful if the parent hybridoma cell is not available for DNA sequencing but also when the amino acid sequence does not match the DNA sequence. You can add Edman sequencing to obtain the heavy and light chain of the monoclonal antibody.

    What is the Sequence Coverage?

    This is the the number of amino acids in a protein sequence that were found in the peptides observed by mass spectrometry.

    I want to identify a protein from an organism where the genome has not been sequenced, is this possible?

    With de-novo peptide sequencing you can obtain the full protein sequence - but cannot identify the protein if it is completely novel. The protein sequencing is performed by nanoLC-MS/MS and database searching with PEAKS and Mascot software.

    Who is this for?

    We primarily help customers within the pharmaceutical industry, and biotechs working on drug development, e.g.

    Project managers

    Working with process development and characterization projects.

    Analytical experts

    Protein scientists that work with ELISA, masss spectrometry, or CMC.

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