Protein Sequencing

Determine amino acid sequence of protein, peptide and mAb

Amino acid sequencing (AA) of proteins, antibodies, and peptides is an important part of detailed sequence analysis. Therefore, the protein sequencing service provided by Alphalyse is based on the original Edman degradation and high-resolution mass spectrometry technology.<...

Amino acid sequencing (AA) of proteins, antibodies, and peptides is an important part of detailed sequence analysis. Therefore, the protein sequencing service provided by Alphalyse is based on the original Edman degradation and high-resolution mass spectrometry technology.

The N-terminal Edman degradation analysis provides up to 40 AA residues from the N-terminal – without any prior knowledge about the protein sequence. The MS technology is mainly used as part of peptide mapping to search specific protein databases but can also be performed without sequence information (de-novo sequencing).

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What is de-novo sequencing?

You can perform de-novo sequencing of monoclonal antibodies using tandem MS (nanoLC-MS/MS) and database searching with PEAKS and Mascot software. The method is especially useful if the parent hybridoma cell is not available for DNA sequencing but can also be utilized when the amino acid sequence does not match the DNA sequence. After the analysis, N-terminal Edman sequencing can be performed to obtain the heavy and light chain of the monoclonal antibody.

What is the Sequence Coverage?

This is the the number of amino acids in a protein sequence that were found in the peptides observed by mass spectrometry.

I want to identify a protein from an organism where the genome has not been sequenced, is this possible?

With de-novo peptide sequencing you can obtain the full protein sequence - but cannot identify the protein if it is completely novel. The sequencing is performed by nanoLC-MS/MS and database searching with PEAKS and Mascot software.

Who is this for?

We primarily help customers within the pharmaceutical industry, and biotechs working on drug development, e.g.

Project managers

Working with process development and characterization projects.

Analytical experts

Protein scientists that work with elisa, masss spectrometry, or CMC.

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