N-terminal Edman sequencing

Edman sequencing of protein or antibody

N-terminal sequencing of protein, is a requirement in the ICH Q6B Guideline and is often done by  Edman degradation. It is however also a natural part of the structural characterization of recombinant proteins – used in clinical testing and to demonstrate comparability/consistency between GMP batches.

Our automatic, cyclic Edman degradation procedure cleaves off the amino acid residue one at a time. This technique allows us to identify each individual amino acid by chromatography. Note, however, that the analysis will not work for samples containing multiple N-terminals and for N-terminally blocked proteins.

Protein n-terminal Edman sequencing service includes:

• Optional 1D SDS gel electrophoresis of the protein and transfer to PVDF membrane
• Sequencing from the n-terminal by Edman
• Determination of up to 30 amino acids
• Comparison of data to expected drug substance sequence

Antibody sequencing service includes:

• 1D SDS PAGE separation of the heavy & light chain
• Blotting onto PVDF membrane and staining
• N-terminal Edman sequencing – up to 20 amino acids for each chain, running separately
• Optional: De-blocking of pyroglutamic acid

Not exactly what you are looking for? Send us a request using the form below so we can adjust the analysis to meet your specific needs, or have a look at our other protein characterization services.