1D & 2D Electrophoresis
5 articlesSpeedy and detailed protein ID of SDS gel bands
Question: Can I identify hundreds of gel bands in a few days? The biopharmaceutical company that I work for, consistently uses SDS...
1D SDS PAGE versus 2D PAGE. Any recommendations?
Question: Should I use 1D or 2D SDS PAGE? I’m interested in isolating and identifying cell surface receptor proteins. They typically have high...
How much protein should I load for protein identification by mass spec
Question: How should I prepare my samples for 2D electrophoresis? How should I prepare my samples for 2D electrophoresis, and how much...
How to increase protein concentration before loading onto 1D SDS PAGE
Question: May I precipitate with TCA prior to loading to SDS gel? Is it ok if I precipitate my samples with TCA...
Protocol for gel silver staining – mass spec & protein identification
Problems arising from using commercial staining kits: Scientists use silver staining for sensitive detection of proteins separated by 1D and 2D...