1D & 2D Electrophoresis

4 articles

Speedy and detailed protein ID of SDS gel bands

Question: Can I identify hundreds of gel bands in a few days? The biopharmaceutical company I work for consistently uses SDS PAGE...

Jun 9. 2020 | Thomas Kofoed |
2D PAGE preparation for MALDI ISD

1D SDS PAGE versus 2D PAGE. Any recommendations?

Question: Should I use 1D or 2D SDS PAGE? I'm interested in isolating and identifying cell surface receptor proteins. In my experience, they...

Sep 18. 2016 | Ejvind Mørtz |

How much protein should I load for protein identification by mass spec

Question: How should I prepare my samples for 2D electrophoresis? How should I prepare my samples for 2D electrophoresis, and how much...

Jun 8. 2016 | Ejvind Mørtz |
Increase protein concentration on PVDF membrane

How to increase protein concentration before loading onto 1D SDS PAGE

Question: May I precipitate with TCA before loading to SDS gel? Is it ok to precipitate my samples with TCA before loading...

Jun 7. 2016 | Ejvind Mørtz |
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