4 articles
2D PAGE preparation for MALDI ISD

1D SDS PAGE versus 2D PAGE. I’m considering 2D electrophoresis, any recommendations?

Question: I’m interested in isolating and identifying cell surface receptor proteins. They typically have high Mw, are glycosylated, and also transmembrane...

Sep 18. 2016 | Ejvind Mørtz | 0

How should I prepare my samples for 2D electrophoresis, and how much protein should I load to get protein identification by mass spec?

How should I prepare my samples for 2D electrophoresis, and how much protein should I load to get protein ID...

Jun 8. 2016 | Ejvind Mørtz | 0
N- and C-terminal sequencing from PVDF membrane

How to increase protein concentration before loading onto 1D SDS PAGE

Question: I have another question:  Is it ok if I precipitate my samples with TCA prior loading to the SDS-Gel? If not, how...

Jun 7. 2016 | Ejvind Mørtz | 0

Protocol for silver staining of gels optimized for mass spectrometry and protein identification

Problems arising from using commercial staining kits: Scientists use silver staining for sensitive detection of proteins separated by 1D and 2D...

Aug 6. 2009 | Ejvind Mørtz | 1
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