1D & 2D Electrophoresis

5 articles

Speedy and detailed protein ID of SDS gel bands

Question: The biopharmaceutical company that I work for, consistently uses SDS PAGE and Western Blotting to check protein expression. But this...

Jun 9. 2020 | Thomas Kofoed |
2D PAGE preparation for MALDI ISD

1D SDS PAGE versus 2D PAGE. Any recommendations?

Question: I’m interested in isolating and identifying cell surface receptor proteins. They typically have high MW, are glycosylated, and have transmembrane domains. I’m...

Sep 18. 2016 | Ejvind Mørtz |

How much protein should I load for protein identification by mass spec

Question: How should I prepare my samples for 2D electrophoresis, and how much protein should I load to get protein ID...

Jun 8. 2016 | Ejvind Mørtz |
Increase protein concentration on PVDF membrane

How to increase protein concentration before loading onto 1D SDS PAGE

Question: Is it ok if I precipitate my samples with TCA prior loading to the SDS-Gel - to increase the protein...

Jun 7. 2016 | Ejvind Mørtz |

Protocol for gel silver staining – mass spec & protein identification

Problems arising from using commercial staining kits: Scientists use silver staining for sensitive detection of proteins separated by 1D and 2D...

Aug 6. 2015 | Ejvind Mørtz |
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