1D & 2D Electrophoresis
4 articles
1D SDS PAGE versus 2D PAGE. Any recommendations?
Question: I’m interested in isolating and identifying cell surface receptor proteins. They typically have high MW, are glycosylated, and have transmembrane domains. I’m...
How much protein should I load for protein identification by mass spec?
Question: How should I prepare my samples for 2D electrophoresis, and how much protein should I load to get protein ID...
How to increase protein concentration before loading onto 1D SDS PAGE
Question: Is it ok if I precipitate my samples with TCA prior loading to the SDS-Gel? If not, how do you...
Protocol for gel silver staining – mass spec & protein identification
Problems arising from using commercial staining kits: Scientists use silver staining for sensitive detection of proteins separated by 1D and 2D...