For N-terminal Edman sequencing SDS PAGE separated proteins can be electroblotted onto PVDF membranes. After PVDF staining You may cut out the protein bands/spots for N-terminal Edman sequencing.
Compatible PVDF staining methods include:
- Ponceau S
- Coomassie Blue
- Amido Black
- Fluorescence Sypro Ruby staining
NB: You can not use Silver staining and Western blots in combination with Edman sequencing!