Effective 1D/2D PVDF staining protocols (for N-terminal sequencing)

Alphalyse
May 24. 2016

For N-terminal Edman sequencing SDS PAGE separated proteins can be electroblotted onto PVDF membranes. After PVDF staining You may cut out the protein bands/spots for N-terminal Edman sequencing.

Compatible PVDF staining methods include:

  • Ponceau S
  • Coomassie Blue
  • Amido Black
  • Fluorescence Sypro Ruby staining

NB: You can not use Silver staining and Western blots in combination with Edman sequencing!

Free protocol for optimal electroblotting & PVDF staining:

PVDF Blotting and Staining Protocols

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