For N-terminal Edman sequencing SDS PAGE you can electro-blot the separated proteins onto PVDF membranes. After PVDF staining you may then cut out the protein bands/spots.
However, not all staining methods are compatible with N-terminal Edman sequencing. Thus, the recommended methods include:
- Ponceau S
- Coomassie Blue
- Amido Black
- Fluorescence Sypro Ruby staining
NB: Silver staining and Western blots do not work in combination with Edman sequencing!
Free protocol for optimal electroblotting & PVDF staining
Below you will find a link to easy (and free) step-by-step protocols for optimized blotting. So far, they have been downloaded more than 7000 times!
Other useful links
In the articles below you can find more information on how to optimize the blotting conditions: