Effective 1D/2D PVDF staining protocols (for N-terminal sequencing)

May 24. 2016

For N-terminal Edman sequencing SDS PAGE you can electro-blot the separated proteins onto PVDF membranes. After PVDF staining you may then cut out the protein bands/spots.

However, not all staining methods are compatible with N-terminal Edman sequencing. Thus, the recommended methods include:

  • Ponceau S
  • Coomassie Blue
  • Amido Black
  • Fluorescence Sypro Ruby staining

NB: Silver staining and Western blots do not work in combination with Edman sequencing!

Free protocol for optimal electroblotting & PVDF staining:

Download PVDF Blotting and Staining Protocols.

N- and C-terminal sequencing from PVDF membrane

Other useful links:

Increase protein concentration before loading onto 1D SDS PAGE

How to prepare samples for 2D electrophoresis

About Alphalyse

Alphalyse uses expertise in the field of protein chemistry and bioinformatics, combined with top-of-the-line mass spectrometry equipment, to provide protein analysis services globally.

Alphalyse aims to deliver high quality data and customer service to all of our clients in a fast and convenient way.


Newsletter Subscribe to The Alphalyse Newsletter and get more
information on protein analysis methods and applications
© 2019 Alphalyse, Inc.