For N-terminal Edman sequencing SDS PAGE you can electro-blot the separated proteins onto PVDF membranes. After PVDF staining you may then cut out the protein bands/spots.
However, not all staining methods are compatible with N-terminal Edman sequencing. Thus, the recommended methods include:
- Ponceau S
- Coomassie Blue
- Amido Black
- Fluorescence Sypro Ruby staining
NB: Silver staining and Western blots do not work in combination with Edman sequencing!
Free protocol for optimal electroblotting & PVDF staining:
Other useful links: