Analysis of Biopharmaceuticals
13 articlesSpeedy and detailed protein ID of SDS gel bands
Question: Can I identify hundreds of gel bands in a few days? The biopharmaceutical company that I work for, consistently uses SDS...
Assessing and monitoring the quality of therapeutic mAb products
Question: Which analyses should be part of mAb characterization? I need to ensure the quality and consistency of my mAbs. Which analyses...
How to characterize antibody glycosylation by HILIC-MS
Question: How do I characterize the N-glycosylation of my antibody? What is N-glycosylation and how do I characterize the N-glycosylation of my...
Simple way to perform label-free quantification of Host Cell Proteins
Question: How can I improve label-free quantification of HCP? I find it difficult to get an accurate label-free quantification of Host Cell...
How to identify peaks observed by UV-HPLC in stability studies
Question: How do we identify the peaks observed by UV-HPLC? We have developed a Reversed-Phase (RP) UV-HPLC chromatography data analysis method. It...
What is SWATH mass spectrometry and its advantages in HCP analysis?
Host Cell Proteins (HCPs) in protein biopharmaceuticals can be analyzed by the mass spectrometry SWATH analysis. It provides both identification...
4 reasons to perform N-terminal protein sequence analysis by Edman
Question: How should I sequence mAbs? How do I obtain the N-terminal sequences of the heavy chain and light chain of my...
Why disulfide bridges are critical quality attributes of biologics
Question: Can disulfide bridges influence the stability of my protein? The activity of my biopharmaceutical protein is low, and I suspect that...
Why you need accurate concentration determination of protein standards
Question: How do I quantify my protein standard? How do I quantify my protein standard? Is it true that it if it...
Determine the Molar Extinction Coefficient of protein in 3 small steps
Question: How do I determine the molar extinction coefficient of my protein? I have a purified protein that I would like to...
Identification of small host cell proteins (small Mw HCPs)
Question: How do I identify HCPs with low molecular weight? I work on a drug substance protein with a mass of 15...
Host Cell Protein monitoring in process development – using mass spec
Mass spectrometry based Host Cell Protein identification and quantification The Mass Spec Host Cell Protein (HCP) assay is applied for monitoring...