Analysis of Biopharmaceuticals
15 articlesSpeedy and detailed protein ID of SDS gel bands
Question: The biopharmaceutical company that I work for, consistently uses SDS PAGE and Western Blotting to check protein expression. But this...
Assessing and monitoring the quality of therapeutic mAb products
Question: I need to ensure the quality and consistency of my mAbs. Which analyses should be conducted for proper mAb characterization? Answer: For...
How to characterize antibody glycosylation by HILIC-MS
Question: What is N-glycosylation and how do I characterize the N-glycosylation of my antibody? Is it possible to compare batch-to-batch variations...
Simple way to perform label-free quantification of Host Cell Proteins
Question: I find it difficult to get an accurate label-free quantification of Host Cell Proteins with traditional multiple reaction monitoring using...
How to identify peaks observed by UV-HPLC in stability studies
Question: We have developed a Reversed-Phase (RP) UV-HPLC chromatography data analysis method. It is used for stability studies of our new...
What is SWATH mass spectrometry and its advantages in HCP analysis?
Host Cell Proteins (HCPs) in protein biopharmaceuticals can be analyzed by the mass spectrometry SWATH analysis. It provides both identification...
4 reasons to perform N-terminal protein sequence analysis by Edman
Question: How do I obtain the N-terminal sequences of the heavy chain and light chain of my monoclonal antibody? Is there...
What purity is required for mAb sequencing by de novo?
Question: My monoclonal antibody is not 100% pure, what is the purity required for mAb sequencing with the de-novo method? Answer: We typically...
How to get 100% antibody sequence coverage
Question: Is it possible to perform a de-novo sequencing of my purified monoclonal antibody without the hybridoma cells? Will I get 100% antibody...
Why disulfide bridges are critical quality attributes of biologics
Question: The activity of my biopharmaceutical protein is low, and I suspect that it is related to decreased protein stability. Could...
Why you need accurate concentration determination of protein standards
Question: How do I quantify my protein standard? Is it true that it if it is done incorrectly it can affect...
Determine the Molar Extinction Coefficient of protein in 3 small steps
Question: I have a purified protein that I would like to quantify accurately in my lab using 280 nm UV measurement...
Identification of small host cell proteins (small Mw HCPs)
Question: I work on a drug substance protein with a mass of 15 kDa. I would expect small host cell protein...
Host Cell Protein monitoring in process development – using mass spec
Mass spectrometry based Host Cell Protein identification and quantification The Mass Spec Host Cell Protein (HCP) assay is applied for monitoring...