Analysis of Biopharmaceuticals

15 articles

Speedy and detailed protein ID of SDS gel bands

Question: The biopharmaceutical company that I work for, consistently uses SDS PAGE and Western Blotting to check protein expression. But this...

Jun 9. 2020 | Thomas Kofoed |
mAb analysis by peptide mapping

Assessing and monitoring the quality of therapeutic mAb products

Question: I need to ensure the quality and consistency of my mAbs. Which analyses should be conducted for proper mAb characterization? Answer: For...

Nov 25. 2019 | Thomas Kofoed |

How to characterize antibody glycosylation by HILIC-MS

Question: What is N-glycosylation and how do I characterize the N-glycosylation of my antibody? Is it possible to compare batch-to-batch variations...

Mar 4. 2019 | Thomas Kofoed |
1000 proteins quantified in e. coli mock lysate - HCP

Simple way to perform label-free quantification of Host Cell Proteins

Question:   I find it difficult to get an accurate label-free quantification of Host Cell Proteins with traditional multiple reaction monitoring using...

Jun 25. 2018 | Ejvind Mørtz |

How do ‘Jackpot HCPs’ and a ‘Lucky Goat’ influence my ELISA results?

Question: I tested two different HCP ELISA kits on my biopharmaceutical. Why do they give different results and which one is...

May 28. 2018 | Anette Draborg |
UV-HPLC data analysis

How to identify peaks observed by UV-HPLC in stability studies

Question:   We have developed a Reversed-Phase (RP) UV-HPLC chromatography data analysis method. It is used for stability studies of our new...

Jan 24. 2018 | Thomas Kofoed |
What is SWATH?

What is SWATH mass spectrometry and its advantages in HCP analysis?

Host Cell Proteins (HCPs) in protein biopharmaceuticals can be analyzed by the mass spectrometry SWATH analysis. It provides both identification...

Jun 22. 2017 | Thomas Kofoed |

4 reasons to perform N-terminal protein sequence analysis by Edman

Question: How do I obtain the N-terminal sequences of the heavy chain and light chain of my monoclonal antibody? Is there...

Apr 26. 2017 | Ejvind Mørtz |
De-novo sequencing mAb

What purity is required for mAb sequencing by de novo?

Question: My monoclonal antibody is not 100% pure, what is the purity required for mAb sequencing with the de-novo method?   Answer: We typically...

Mar 20. 2017 | Fen Yang Reske-Nielsen |
100% sequence coverage map of antibody

How to get 100% antibody sequence coverage

Question: Is it possible to perform a de-novo sequencing of my purified monoclonal antibody without the hybridoma cells? Will I get 100% antibody...

Mar 17. 2017 | Fen Yang Reske-Nielsen |

Why disulfide bridges are critical quality attributes of biologics

Question: The activity of my biopharmaceutical protein is low, and I suspect that it is related to decreased protein stability. Could...

Mar 7. 2017 | Thomas Kofoed |

Why you need accurate concentration determination of protein standards

Question: How do I quantify my protein standard? Is it true that it if it is done incorrectly it can affect...

Jan 18. 2017 | Thomas Kofoed |
Extinction coefficient protein concentration

Determine the Molar Extinction Coefficient of protein in 3 small steps

Question: I have a purified protein that I would like to quantify accurately in my lab using 280 nm UV measurement...

Nov 15. 2016 | Thanh Ha Nguyen |
Analysis method for Mw HCPs (small HCPs) below 20 kDa

Identification of small host cell proteins (small Mw HCPs)

Question: I work on a drug substance protein with a mass of 15 kDa. I would expect small host cell protein...

Oct 11. 2016 | Ejvind Mørtz |

Host Cell Protein monitoring in process development – using mass spec

Mass spectrometry based Host Cell Protein identification and quantification The Mass Spec Host Cell Protein (HCP) assay is applied for monitoring...

Oct 2. 2009 | Ejvind Mørtz |
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