Analysis of Biopharmaceuticals
10 articlesSpeedy and detailed protein ID of SDS gel bands
Question: Can I identify hundreds of gel bands in a few days? The biopharmaceutical company that I work for consistently uses SDS...
Assessing and monitoring the quality of therapeutic mAb products
Question: Which analyses should be part of mAb characterization? I need to ensure the quality and the consistency of my mAbs. Do...
Simple way to perform label-free quantification of Host Cell Proteins
Question: How can I improve label-free quantification of HCP? I find it difficult to get an accurate label-free quantification of Host Cell...
How do ‘Jackpot HCPs’ and a ‘Lucky Goat’ influence my ELISA results?
Question: Why do HPC ELISAs give different results? I tested two different HCP ELISA kits on my biopharmaceutical. Why do they give...
How to identify peaks observed by UV-HPLC in stability studies
Question: How do we identify the peaks observed by UV-HPLC? We have developed a Reversed-Phase (RP) UV-HPLC chromatography data analysis method for...
What is SWATH mass spectrometry and its advantages?
The mass spectrometry SWATH analysis can analyze host Cell Proteins (HCPs) in protein biopharmaceuticals. It provides both identification and quantification...
Why you need accurate concentration determination of protein standards
Question: How do I quantify my protein standard? How do I quantify my protein standard? Is it true that it if it...
Determine the Molar Extinction Coefficient of protein in 3 small steps
Question: How do I determine the molar extinction coefficient of my protein? I have a purified protein that I would like to...
Identification of small host cell proteins (small Mw HCPs)
Question: How do I identify HCPs with low molecular weight? I work on a drug substance protein with a mass of 15...
Host Cell Protein monitoring in process development – using mass spec
Mass spectrometry based Host Cell Protein identification and quantification The Mass Spec Host Cell Protein (HCP) assay is applied for monitoring...