mAb analysis
6 articles
Assessing and monitoring the quality of therapeutic mAb products
Question: I need to ensure the quality and consistency of my mAbs. Which analyses should be conducted for proper mAb characterization? Answer: For...
How to characterize antibody glycosylation by HILIC-MS
Question: What is N-glycosylation and how do I characterize the N-glycosylation of my antibody? Is it possible to compare batch-to-batch variations...
4 reasons to perform N-terminal protein sequence analysis by Edman
Question: How do I obtain the N-terminal sequences of the heavy chain and light chain of my monoclonal antibody? Is there...
What purity is required for mAb sequencing by de novo?
Question: My monoclonal antibody is not 100% pure, what is the purity required for mAb sequencing with the de-novo method? Answer: We typically...
How to get 100% antibody sequence coverage
Question: Is it possible to perform a de-novo sequencing of my purified monoclonal antibody without the hybridoma cells? Will I get 100% antibody...
Why disulfide bridges are critical quality attributes of biologics
Question: The activity of my biopharmaceutical protein is low, and I suspect that it is related to decreased protein stability. Could...