mAb analysis

6 articles
mAb analysis by peptide mapping

Assessing and monitoring the quality of therapeutic mAb products

Question: I need to ensure the quality and consistency of my mAbs. Which analyses should be conducted for proper mAb characterization? Answer: For...

Nov 25. 2019 | Thomas Kofoed |

How to characterize antibody glycosylation by HILIC-MS

Question: What is N-glycosylation and how do I characterize the N-glycosylation of my antibody? Is it possible to compare batch-to-batch variations...

Mar 4. 2019 | Thomas Kofoed |

4 reasons to perform N-terminal protein sequence analysis by Edman

Question: How do I obtain the N-terminal sequences of the heavy chain and light chain of my monoclonal antibody? Is there...

Apr 26. 2017 | Ejvind Mørtz |
De-novo sequencing mAb

What purity is required for mAb sequencing by de novo?

Question: My monoclonal antibody is not 100% pure, what is the purity required for mAb sequencing with the de-novo method?   Answer: We typically...

Mar 20. 2017 | Fen Yang Reske-Nielsen |
100% sequence coverage map of antibody

De-novo Sequencing: How to get 100% antibody sequence coverage

Question: Is it possible to perform a de-novo sequencing of my purified monoclonal antibody without the hybridoma cells? Will I get 100% antibody...

Mar 17. 2017 | Fen Yang Reske-Nielsen |

Why disulfide bridges are critical quality attributes of biologics

Question: The activity of my biopharmaceutical protein is low, and I suspect that it is related to decreased protein stability. Could...

Mar 7. 2017 | Thomas Kofoed |
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