mAb analysis
5 articlesHow to characterize antibody glycosylation by HILIC-MS
Question: What is N-glycosylation and how do I characterize the N-glycosylation of my antibody? Is it possible to compare batch-to-batch variations? Answer: Glycosylation is...
4 reasons why N-terminal sequencing should be done by Edman degradation
Question: How do I obtain the N-terminal sequences of the heavy chain and light chain of my monoclonal antibody? Answer: mAb N-terminal sequence...
What purity is required for de-novo sequencing of my antibody?
Question: My monoclonal antibody is not 100% pure, what is the purity required for de-novo sequencing? Answer: We typically observe two types of protein impurities in...
Antibody de-novo Sequencing: How to get 100% sequence coverage
Question: Is it possible to perform a de-novo sequencing of my purified monoclonal antibody without the hybridoma cells? Answer: Yes! – Your purified antibody can...
Why disulfide bridges are critical quality attributes of biologics
Question: The activity of my biopharmaceutical protein is low, and I suspect that it is related to decreased protein stability. What...