N- and C-Terminal Protein Sequencing

6 articles

Effective 1D/2D PVDF staining protocols (for N-terminal sequencing)

For N-terminal Edman sequencing SDS PAGE you can electro-blot the separated proteins onto PVDF membranes. After PVDF staining you may...

May 24. 2016 | Ejvind Mørtz |

Why is my protein’s N-terminal blocked (and what can I do about it)?

N-terminal sequencing is a requirement according to the ICH Q6B Guideline. This includes structural characterization of recombinant proteins for clinical...

Aug 31. 2015 | Ejvind Mørtz |

Preparing samples for N- and C-terminal sequencing by MALDI ISD?

Question: I am interested in your N and C-terminal sequencing using MALDI ISD. How do I prepare the sample for...

Feb 22. 2014 | Ejvind Mørtz |

Optimize electroblotting on PVDF membrane for N-terminal Edman sequencing

How do I optimize electroblotting conditions onto PVDF membrane for N-terminal Edman sequencing? Our 12 kDa protein separates well on...

Oct 13. 2013 | Ejvind Mørtz |
N- and C-terminal Sequencing

3 Techniques for N- and C-terminal sequencing of proteins

Where does the amino acid sequence start and where does it end? The terminal amino acid sequences are important for the...

Sep 16. 2013 | Ejvind Mørtz |

N- and C-terminal protein sequencing using MALDI ISD

Structural characterization of purified natural and recombinant proteins traditionally includes N-terminal Edman sequencing to confirm 5-15 amino acid residues from...

Aug 18. 2013 | Ejvind Mørtz |
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