N- and C-Terminal Protein Sequencing
5 articlesWhy is my protein’s N-terminal blocked (and what can I do about it)?
Question: I found that the N-terminal of my protein is blocked. How can I then analyze it by Edman degradation/sequencing? Answer: N-terminal sequencing...
3 Techniques for N- and C-terminal sequencing of proteins
Question: Where does the amino acid sequence start and where does it end? Can you describe relevant sequencing techniques? Answer: The terminal amino...
Preparing samples for N- and C-terminal sequencing by MALDI ISD
Question: I am interested in your N and C-terminal sequencing using MALDI ISD. How do I prepare the sample for analysis? Answer: MALDI...
Optimize electroblotting on PVDF membrane for N-terminal Edman sequencing
Question: How do I optimize electroblotting conditions onto PVDF membrane for N-terminal Edman sequencing? Our 12 kDa protein separates well on 1D...
N- and C-terminal protein sequencing using MALDI ISD
Structural characterization of purified natural and recombinant proteins traditionally includes N-terminal Edman sequencing to confirm 5-15 amino acid residues from...