N- and C-Terminal Protein Sequencing

5 articles

Why is my protein’s N-terminal blocked (and what can I do about it)?

Question:   I found that the N-terminal of my protein is blocked. How can I then analyze it by Edman degradation/sequencing?   Answer:   N-terminal sequencing...

Aug 31. 2015 | Ejvind Mørtz |

Preparing samples for N- and C-terminal sequencing by MALDI ISD

Question:   I am interested in your N and C-terminal sequencing using MALDI ISD. How do I prepare the sample for analysis?   Answer:   MALDI...

Feb 22. 2014 | Ejvind Mørtz |

Optimize electroblotting on PVDF membrane for N-terminal Edman sequencing

Question:   How do I optimize electroblotting conditions onto PVDF membrane for N-terminal Edman sequencing? Our 12 kDa protein separates well on 1D...

Oct 13. 2013 | Ejvind Mørtz |
N- and C-terminal Sequencing

3 Techniques for N- and C-terminal sequencing of proteins

Question:   Where does the amino acid sequence start and where does it end? Can you describe relevant sequencing techniques?   Answer:   The terminal amino...

Sep 16. 2013 | Ejvind Mørtz |

N- and C-terminal protein sequencing using MALDI ISD

Structural characterization of purified natural and recombinant proteins traditionally includes N-terminal Edman sequencing to confirm 5-15 amino acid residues from...

Aug 18. 2013 | Ejvind Mørtz |
Newsletter Subscribe to The Alphalyse Newsletter and get more
information on protein analysis methods and applications
© 2019 Alphalyse, Inc.