N- and C-Terminal Protein Sequencing
4 articlesWhy is my protein’s N-terminal blocked (and what can I do about it)?
Question: Does a blocked N-terminal hinder Edman degradation? I found that the N-terminal of my protein is blocked. So, how can I...
3 Techniques to find the protein N- and C-terminal sequence
Question: How do I sequence the protein n-terminal and c-terminal? Where does the amino acid sequence start and where does it end?...
Preparing samples for N- and C-terminal sequencing by MALDI ISD
Question: How do I prepare samples for MALDI ISD analysis? I am interested in your N and C-terminal sequencing using MALDI ISD....
Optimize PVDF membrane electroblotting for N-terminal Edman sequencing
Question: How do I optimize electroblotting conditions onto a PVDF membrane? Our 12 kDa protein separates well on 1D SDS PAGE but...