N- and C-Terminal Protein Sequencing

4 articles

Why is my protein’s N-terminal blocked (and what can I do about it)?

Question: Does a blocked N-terminal hinder Edman degradation? I found that the N-terminal of my protein is blocked. So, how can I...

Aug 31. 2017 | Ejvind Mørtz |
Protein N-terminal and C-terminal sequencing of proteins

3 Techniques to find the protein N- and C-terminal sequence

Question: How do I sequence the protein n-terminal and c-terminal? Where does the amino acid sequence start and where does it end?...

Sep 16. 2016 | Ejvind Mørtz |

Preparing samples for N- and C-terminal sequencing by MALDI ISD

Question: How do I prepare samples for MALDI ISD analysis? I am interested in your N and C-terminal sequencing using MALDI ISD....

Feb 22. 2014 | Ejvind Mørtz |

Optimize PVDF membrane electroblotting for N-terminal Edman sequencing

Question: How do I optimize electroblotting conditions onto a PVDF membrane? Our 12 kDa protein separates well on 1D SDS PAGE but...

Oct 13. 2013 | Ejvind Mørtz |
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