N- and C-Terminal Protein Sequencing

4 articles

Why is my protein’s N-terminal blocked (and what can I do about it)?

Question: I found that the N-terminal of my protein is blocked. How can I then analyze it by Edman degradation /...

Aug 31. 2017 | Ejvind Mørtz |
Protein N-terminal and C-terminal sequencing of proteins

3 Techniques for protein N- and C-terminal sequencing

Question: Where does the amino acid sequence start and where does it end? Can you describe relevant sequencing techniques from the...

Sep 16. 2016 | Ejvind Mørtz |

Preparing samples for N- and C-terminal sequencing by MALDI ISD

Question: I am interested in your N and C-terminal sequencing using MALDI ISD. How do I prepare the sample for analysis? Answer: MALDI...

Feb 22. 2014 | Ejvind Mørtz |

Optimize PVDF membrane electroblotting for N-terminal Edman sequencing

Question: How do I optimize electroblotting conditions onto PVDF membrane for N-terminal Edman sequencing? Our 12 kDa protein separates well on 1D...

Oct 13. 2013 | Ejvind Mørtz |
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