Effective 1D/2D PVDF staining protocols (for N-terminal sequencing)
For N-terminal Edman sequencing SDS PAGE separated proteins can be electroblotted onto PVDF membranes. After PVDF staining You may cut...
Why is my protein’s N-terminal blocked (and what can I do about it)?
N-terminal sequencing is a requirement according to the ICH Q6B Guideline. This includes structural characterization of recombinant proteins for clinical...
Preparing samples for N- and C-terminal sequencing by MALDI ISD?
Question: I am interested in your N and C-terminal sequencing using MALDI ISD. How do I prepare the sample for...
How to optimize electroblotting conditions onto PVDF membrane for N-terminal Edman sequencing
How do I optimize electroblotting conditions onto PVDF membrane for N-terminal Edman sequencing? Our 12 kDa protein separates well on...
3 Techniques for N- and C-terminal sequencing of proteins
Where does the amino acid sequence start and where does it end? The terminal amino acid sequences are important for the...
N- and C-terminal protein sequencing using MALDI ISD
Structural characterization of purified natural and recombinant proteins traditionally includes N-terminal Edman sequencing to confirm 5-15 amino acid residues from...