Why is my protein’s N-terminal blocked (and what can I do about it)?

Question: I found that the N-terminal of my protein is blocked. How can I then analyze it by Edman degradation /...

Aug 31. 2017 | Ejvind Mørtz |
Amino Acid analysis

4 things to remember about Amino Acid Analysis of proteins & peptides

Why perform Amino Acid Analysis? Amino acid analysis of proteins is a method to determine the absolute amounts of individual amino...

Sep 23. 2016 | Ejvind Mørtz |
2D PAGE preparation for MALDI ISD

1D SDS PAGE versus 2D PAGE. Any recommendations?

Question: I’m interested in isolating and identifying cell surface receptor proteins. They typically have high MW, are glycosylated, and have transmembrane domains. I’m...

Sep 18. 2016 | Ejvind Mørtz |

How much protein should I load for protein identification by mass spec

Question: How should I prepare my samples for 2D electrophoresis, and how much protein should I load to get protein ID...

Jun 8. 2016 | Ejvind Mørtz |
Mass spectrometry analysis of protein

Common protein contaminants in mass spectrometric protein ID

Questions: Is the keratin identified by the protein ID service in my 1D gel band a contamination? Or is it...

Jun 3. 2016 | Ejvind Mørtz |

Preparing samples for N- and C-terminal sequencing by MALDI ISD

Question: I am interested in your N and C-terminal sequencing using MALDI ISD. How do I prepare the sample for analysis? Answer: MALDI...

Feb 22. 2014 | Ejvind Mørtz |
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