Why is my protein’s N-terminal blocked (and what can I do about it)?

Question: I found that the N-terminal of my protein is blocked. How can I then analyze it by Edman degradation /...

Aug 31. 2017 | Ejvind Mørtz |

4 reasons to perform N-terminal protein sequence analysis by Edman

Question: How do I obtain the N-terminal sequences of the heavy chain and light chain of my monoclonal antibody? Is there...

Apr 26. 2017 | Ejvind Mørtz |

Optimize PVDF membrane electroblotting for N-terminal Edman sequencing

Question: How do I optimize electroblotting conditions onto PVDF membrane for N-terminal Edman sequencing? Our 12 kDa protein separates well on 1D...

Oct 13. 2013 | Ejvind Mørtz |
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