Why is my protein’s N-terminal blocked (and what can I do about it)?

Question: Does a blocked N-terminal hinder Edman degradation? I found that the N-terminal of my protein is blocked. So, how can I...

Aug 31. 2017 | Ejvind Mørtz |

4 reasons to perform N-terminal protein sequence analysis by Edman

Question: How should I sequence mAbs? How do I obtain the N-terminal sequences of the heavy chain and light chain of my...

Apr 26. 2017 | Ejvind Mørtz |

Optimize PVDF membrane electroblotting for N-terminal Edman sequencing

Question: How do I optimize electroblotting conditions onto a PVDF membrane? Our 12 kDa protein separates well on 1D SDS PAGE but...

Oct 13. 2013 | Ejvind Mørtz |
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