Analysis method for Mw HCPs (small HCPs) below 20 kDa

Identification of small host cell proteins (low Mw HCPs)

Question:   Could you suggest an analysis method to detect low Mw HCPs (small HCPs) below 20 kDa? I work on a drug...

Oct 11. 2016 | Ejvind Mørtz |

How much protein should I load for protein identification by mass spec

Question:   How should I prepare my samples for 2D electrophoresis, and how much protein should I load to get protein ID...

Jun 8. 2016 | Ejvind Mørtz |
Preparation of sample for MALDI

What buffer to use for MALDI mass spectrometry analysis of a protein?

Question:   What buffer should I use for MALDI Mass Spectrometry analysis of my protein sample?   Answer:   In general, MALDI-MS is more tolerant to contamination...

Apr 28. 2016 | Ejvind Mørtz |

Preparing samples for N- and C-terminal sequencing by MALDI ISD

Question:   I am interested in your N and C-terminal sequencing using MALDI ISD. How do I prepare the sample for analysis?   Answer:   MALDI...

Feb 22. 2014 | Ejvind Mørtz |

N- and C-terminal protein sequencing using MALDI ISD

Structural characterization of purified natural and recombinant proteins traditionally includes N-terminal Edman sequencing to confirm 5-15 amino acid residues from...

Aug 18. 2013 | Ejvind Mørtz |

Host Cell Protein monitoring & control in process development

Mass spectrometry based Host Cell Protein identification and quantification   The Mass Spec Host Cell Protein (HCP) assay is applied for monitoring...

Oct 2. 2009 | Ejvind Mørtz |
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