How much protein should I load for protein identification by mass spec

Question:   How should I prepare my samples for 2D electrophoresis, and how much protein should I load to get protein ID...

Jun 8. 2016 | Ejvind Mørtz |
Mass spectrometry analysis of protein

Common protein contaminants in mass spectrometric protein ID

Questions: Is the keratin identified by the protein ID service in my 1D gel band a contamination? Or is it...

Jun 3. 2016 | Ejvind Mørtz |
Preparation of sample for MALDI

What buffer to use for MALDI mass spectrometry analysis of a protein?

Question:   What buffer should I use for MALDI Mass Spectrometry analysis of my protein sample?   Answer:   In general, MALDI-MS is more tolerant to contamination...

Apr 28. 2016 | Ejvind Mørtz |

Useful Protein Molecular Weight Calculator kDa

Protein molecular weight calculator kDa   The rule of thumb for conversion of microgram amounts into picomole amounts at different protein molecular...

Nov 10. 2015 | Ejvind Mørtz |

What does the Mascot Score mean?

Question:   I just got my protein identification report. What does the Mascot Score mean?   Answer:   The Protein Identification report contains a list of proteins that have...

Oct 9. 2015 | Ejvind Mørtz |

Protocol for gel silver staining – mass spec & protein identification

Problems arising from using commercial staining kits: Scientists use silver staining for sensitive detection of proteins separated by 1D and 2D...

Aug 6. 2015 | Ejvind Mørtz |

Preparing samples for N- and C-terminal sequencing by MALDI ISD

Question:   I am interested in your N and C-terminal sequencing using MALDI ISD. How do I prepare the sample for analysis?   Answer:   MALDI...

Feb 22. 2014 | Ejvind Mørtz |

N- and C-terminal protein sequencing using MALDI ISD

Structural characterization of purified natural and recombinant proteins traditionally includes N-terminal Edman sequencing to confirm 5-15 amino acid residues from...

Aug 18. 2013 | Ejvind Mørtz |

Host Cell Protein monitoring & control in process development

Mass spectrometry based Host Cell Protein identification and quantification   The Mass Spec Host Cell Protein (HCP) assay is applied for monitoring...

Oct 2. 2009 | Ejvind Mørtz |
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