Why is my protein’s N-terminal blocked (and what can I do about it)?

Question: I found that the N-terminal of my protein is blocked. How can I then analyze it by Edman degradation /...

Aug 31. 2017 | Ejvind Mørtz |
De-novo sequencing mAb

What purity is required for mAb sequencing by de novo?

Question: My monoclonal antibody is not 100% pure, what is the purity required for mAb sequencing with the de-novo method?   Answer: We typically...

Mar 20. 2017 | Fen Yang Reske-Nielsen |
100% sequence coverage map of antibody

De-novo Sequencing: How to get 100% antibody sequence coverage

Question: Is it possible to perform a de-novo sequencing of my purified monoclonal antibody without the hybridoma cells? Will I get 100% antibody...

Mar 17. 2017 | Fen Yang Reske-Nielsen |

Preparing samples for N- and C-terminal sequencing by MALDI ISD

Question: I am interested in your N and C-terminal sequencing using MALDI ISD. How do I prepare the sample for analysis? Answer: MALDI...

Feb 22. 2014 | Ejvind Mørtz |

Optimize PVDF membrane electroblotting for N-terminal Edman sequencing

Question: How do I optimize electroblotting conditions onto PVDF membrane for N-terminal Edman sequencing? Our 12 kDa protein separates well on 1D...

Oct 13. 2013 | Ejvind Mørtz |
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