Why is my protein’s N-terminal blocked (and what can I do about it)?

Question:   I found that the N-terminal of my protein is blocked. How can I then analyze it by Edman degradation/sequencing?   Answer:   N-terminal sequencing...

Aug 31. 2017 | Ejvind Mørtz |
Application note - mAb

What purity is required for de-novo sequencing of my antibody?

Question:   My monoclonal antibody is not 100% pure, what is the purity required for de-novo sequencing?     Answer:   We typically observe two types of protein impurities in...

Mar 20. 2017 | Fen Yang Reske-Nielsen |
100% sequence coverage map of antibody

Antibody de-novo Sequencing: How to get 100% sequence coverage

Question: Is it possible to perform a de-novo sequencing of my purified monoclonal antibody without the hybridoma cells? Answer: Yes! – Your purified antibody can...

Mar 17. 2017 | Fen Yang Reske-Nielsen |

Preparing samples for N- and C-terminal sequencing by MALDI ISD

Question:   I am interested in your N and C-terminal sequencing using MALDI ISD. How do I prepare the sample for analysis?   Answer:   MALDI...

Feb 22. 2014 | Ejvind Mørtz |

Optimize PVDF membrane electroblotting for N-terminal Edman sequencing

Question:   How do I optimize electroblotting conditions onto PVDF membrane for N-terminal Edman sequencing? Our 12 kDa protein separates well on 1D...

Oct 13. 2013 | Ejvind Mørtz |
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