Why you need accurate concentration determination of protein standards
Question: How do I quantify my protein standard? Answer: Have you ever questioned the quantitative data you get from Bradford, Lowry or BCA...
4 things to remember about Amino Acid Analysis of proteins & peptides
Why perform Amino Acid Analysis? Amino acid analysis is a method to determine the absolute amounts of individual amino acids in...
What buffer to use for MALDI mass spectrometry analysis of a protein?
Question: What buffer should I use for MALDI Mass Spectrometry analysis of my protein sample? Answer: In general, MALDI-MS is more tolerant to contamination...
Useful Protein Molecular Weight Calculator kDa
Protein molecular weight calculator kDa The rule of thumb for conversion of microgram amounts into picomole amounts at different protein molecular...
Protocol for gel silver staining – mass spec & protein identification
Problems arising from using commercial staining kits: Scientists use silver staining for sensitive detection of proteins separated by 1D and 2D...
N- and C-terminal protein sequencing using MALDI ISD
Structural characterization of purified natural and recombinant proteins traditionally includes N-terminal Edman sequencing to confirm 5-15 amino acid residues from...