Why you need accurate concentration determination of protein standards
Question: How do I quantify my protein standard? Is it true that it if it is done incorrectly it can affect...
4 things to remember about Amino Acid Analysis of proteins & peptides
Why perform Amino Acid Analysis? Amino acid analysis of proteins is a method to determine the absolute amounts of individual amino...
What buffer to use for MALDI mass spectrometry analysis of a protein?
Question: What buffer should I use for MALDI Mass Spectrometry analysis of my protein sample? Answer: In general, MALDI-MS is more tolerant to contamination...
Useful Protein Molecular Weight Calculator kDa
Protein molecular weight calculator kDaThe rule of thumb for conversion of microgram amounts into picomole amounts at different protein molecular...
Protocol for gel silver staining – mass spec & protein identification
Problems arising from using commercial staining kits: Scientists use silver staining for sensitive detection of proteins separated by 1D and 2D...