Why you need accurate concentration determination of protein standards

Question:   How do I quantify my protein standard?   Answer:   Have you ever questioned the quantitative data you get from Bradford, Lowry or BCA...

Jan 18. 2017 | Thomas Kofoed |
Amino Acid analysis

4 things to remember about Amino Acid Analysis of proteins & peptides

Why perform Amino Acid Analysis?   Amino acid analysis is a method to determine the absolute amounts of individual amino acids in...

Sep 23. 2016 | Ejvind Mørtz |
Preparation of sample for MALDI

What buffer to use for MALDI mass spectrometry analysis of a protein?

Question:   What buffer should I use for MALDI Mass Spectrometry analysis of my protein sample?   Answer:   In general, MALDI-MS is more tolerant to contamination...

Apr 28. 2016 | Ejvind Mørtz |

Useful Protein Molecular Weight Calculator kDa

Protein molecular weight calculator kDa   The rule of thumb for conversion of microgram amounts into picomole amounts at different protein molecular...

Nov 10. 2015 | Ejvind Mørtz |

Protocol for gel silver staining – mass spec & protein identification

Problems arising from using commercial staining kits: Scientists use silver staining for sensitive detection of proteins separated by 1D and 2D...

Aug 6. 2015 | Ejvind Mørtz |

N- and C-terminal protein sequencing using MALDI ISD

Structural characterization of purified natural and recombinant proteins traditionally includes N-terminal Edman sequencing to confirm 5-15 amino acid residues from...

Aug 18. 2013 | Ejvind Mørtz |
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