Why is my protein’s N-terminal blocked (and what can I do about it)?

Question:   I found that the N-terminal of my protein is blocked. How can I then analyze it by Edman degradation/sequencing?   Answer:   N-terminal sequencing...

Aug 31. 2017 | Ejvind Mørtz |
Amino Acid analysis

4 things to remember about Amino Acid Analysis of proteins & peptides

Why perform Amino Acid Analysis?   Amino acid analysis is a method to determine the absolute amounts of individual amino acids in...

Sep 23. 2016 | Ejvind Mørtz |
2D PAGE preparation for MALDI ISD

1D SDS PAGE versus 2D PAGE. Any recommendations?

Question:   I’m interested in isolating and identifying cell surface receptor proteins. They typically have high MW, are glycosylated, and have transmembrane domains. I’m...

Sep 18. 2016 | Ejvind Mørtz |

Useful Protein Molecular Weight Calculator kDa

Protein molecular weight calculator kDa   The rule of thumb for conversion of microgram amounts into picomole amounts at different protein molecular...

Nov 10. 2015 | Ejvind Mørtz |

Protocol for gel silver staining – mass spec & protein identification

Problems arising from using commercial staining kits: Scientists use silver staining for sensitive detection of proteins separated by 1D and 2D...

Aug 6. 2015 | Ejvind Mørtz |

Optimize electroblotting on PVDF membrane for N-terminal Edman sequencing

Question:   How do I optimize electroblotting conditions onto PVDF membrane for N-terminal Edman sequencing? Our 12 kDa protein separates well on 1D...

Oct 13. 2013 | Ejvind Mørtz |
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