Why is my protein’s N-terminal blocked (and what can I do about it)?

Question: Does a blocked N-terminal hinder Edman degradation? I found that the N-terminal of my protein is blocked. So, how can I...

Aug 31. 2017 | Ejvind Mørtz |
Amino Acid analysis

4 things to remember about Amino Acid Analysis of proteins & peptides

Why perform Amino Acid Analysis? Amino acid analysis of proteins is a method to determine the absolute amounts of individual amino...

Sep 23. 2016 | Ejvind Mørtz |
2D PAGE preparation for MALDI ISD

1D SDS PAGE versus 2D PAGE. Any recommendations?

Question: Should I use 1D or 2D SDS PAGE? I’m interested in isolating and identifying cell surface receptor proteins. They typically have high...

Sep 18. 2016 | Ejvind Mørtz |

Useful Protein Molecular Weight Calculator kDa

Protein molecular weight calculator kDa The rule of thumb for conversion of microgram amounts into picomole amounts at different protein molecular...

Nov 10. 2015 | Ejvind Mørtz |

Protocol for gel silver staining – mass spec & protein identification

Problems arising from using commercial staining kits: Scientists use silver staining for sensitive detection of proteins separated by 1D and 2D...

Aug 6. 2015 | Ejvind Mørtz |

Optimize PVDF membrane electroblotting for N-terminal Edman sequencing

Question: How do I optimize electroblotting conditions onto a PVDF membrane? Our 12 kDa protein separates well on 1D SDS PAGE but...

Oct 13. 2013 | Ejvind Mørtz |
Newsletter Subscribe to The Alphalyse Newsletter and get more
information on protein analysis methods and applications
© 2021 Alphalyse, Inc.