Why is my protein’s N-terminal blocked (and what can I do about it)?

Question:   I found that the N-terminal of my protein is blocked. How can I then analyze it by Edman degradation/sequencing?   Answer:   N-terminal sequencing...

Aug 31. 2017 | Ejvind Mørtz |

Why you need accurate concentration determination of protein standards

Question:   How do I quantify my protein standard?   Answer:   Have you ever questioned the quantitative data you get from Bradford, Lowry or BCA...

Jan 18. 2017 | Thomas Kofoed |
Extinction coefficient protein concentration

Find the Molar Extinction Coefficient of your protein in 3 small steps

Question:   I have a purified protein that I would like to quantify accurately in my lab using 280 nm UV measurement...

Nov 15. 2016 | Thanh Ha Nguyen |
Amino Acid analysis

4 things to remember about Amino Acid Analysis of proteins & peptides

Why perform Amino Acid Analysis?   Amino acid analysis is a method to determine the absolute amounts of individual amino acids in...

Sep 23. 2016 | Ejvind Mørtz |
2D PAGE preparation for MALDI ISD

1D SDS PAGE versus 2D PAGE. Any recommendations?

Question:   I’m interested in isolating and identifying cell surface receptor proteins. They typically have high MW, are glycosylated, and have transmembrane domains. I’m...

Sep 18. 2016 | Ejvind Mørtz |

How much protein should I load for protein identification by mass spec

Question:   How should I prepare my samples for 2D electrophoresis, and how much protein should I load to get protein ID...

Jun 8. 2016 | Ejvind Mørtz |
Mass spectrometry analysis of protein

Common protein contaminants in mass spectrometric protein ID

Questions: Is the keratin identified by the protein ID service in my 1D gel band a contamination? Or is it...

Jun 3. 2016 | Ejvind Mørtz |

Useful Protein Molecular Weight Calculator kDa

Protein molecular weight calculator kDa   The rule of thumb for conversion of microgram amounts into picomole amounts at different protein molecular...

Nov 10. 2015 | Ejvind Mørtz |

What does the Mascot Score mean?

Question:   I just got my protein identification report. What does the Mascot Score mean?   Answer:   The Protein Identification report contains a list of proteins that have...

Oct 9. 2015 | Ejvind Mørtz |

Protocol for gel silver staining – mass spec & protein identification

Problems arising from using commercial staining kits: Scientists use silver staining for sensitive detection of proteins separated by 1D and 2D...

Aug 6. 2015 | Ejvind Mørtz |

Preparing samples for N- and C-terminal sequencing by MALDI ISD

Question:   I am interested in your N and C-terminal sequencing using MALDI ISD. How do I prepare the sample for analysis?   Answer:   MALDI...

Feb 22. 2014 | Ejvind Mørtz |

Optimize PVDF membrane electroblotting for N-terminal Edman sequencing

Question:   How do I optimize electroblotting conditions onto PVDF membrane for N-terminal Edman sequencing? Our 12 kDa protein separates well on 1D...

Oct 13. 2013 | Ejvind Mørtz |

N- and C-terminal protein sequencing using MALDI ISD

Structural characterization of purified natural and recombinant proteins traditionally includes N-terminal Edman sequencing to confirm 5-15 amino acid residues from...

Aug 18. 2013 | Ejvind Mørtz |

Host Cell Protein monitoring & control in process development

Mass spectrometry based Host Cell Protein identification and quantification   The Mass Spec Host Cell Protein (HCP) assay is applied for monitoring...

Oct 2. 2009 | Ejvind Mørtz |
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