Protein calculator

GPMAW lite – Protein Molecular Weight Calculator (and much more)

Simple bioinformatics calculations on specific protein sequences   When working with proteins you often need to make simple bioinformatics calculations on a specific...

Apr 10. 2017 | Thomas Kofoed |

How much protein should I load for protein identification by mass spec?

Question:   How should I prepare my samples for 2D electrophoresis, and how much protein should I load to get protein ID...

Jun 8. 2016 | Ejvind Mørtz |
Mass spectrometry analysis of protein

Common protein contaminants in mass spectrometric protein ID

Questions: Is the keratin identified by the protein ID service in my 1D gel band a contamination? Or is it...

Jun 3. 2016 | Ejvind Mørtz |
Eppendorf tubes with protein

Mass spectrometric identification of proteins from Western blots

Question:   We have an unknown protein recognized in Western blot by autoantibodies that we need to identify. Is it possible to send...

May 2. 2016 | Ejvind Mørtz |
Preparation of sample for MALDI

What buffer to use for MALDI mass spectrometry analysis of a protein?

Question:   What buffer should I use for MALDI Mass Spectrometry analysis of my protein sample?   Answer:   In general, MALDI-MS is more tolerant to contamination...

Apr 28. 2016 | Ejvind Mørtz |

What does the Mascot Score mean?

Question:   I just got my protein identification report. What does the Mascot Score mean?   Answer:   The Protein Identification report contains a list of proteins that have...

Oct 9. 2015 | Ejvind Mørtz |

Protocol for gel silver staining – mass spec & protein identification

Problems arising from using commercial staining kits: Scientists use silver staining for sensitive detection of proteins separated by 1D and 2D...

Aug 6. 2015 | Ejvind Mørtz |

Identification of proteolytic degradation of recombinant protein

Question:   I purified a recombinant protein and analyzed it by 1D SDS PAGE. In addition to the intact protein (52 kDa),...

May 9. 2015 | Ejvind Mørtz |

Guide to identification of novel proteins not in public databases

Question:   I recently used Alphalyse's protein identification service to analyze my protein. The sample gave me very good MS data. However it...

Dec 2. 2014 | Ejvind Mørtz |

Host Cell Protein monitoring & control in process development

Mass spectrometry based Host Cell Protein identification and quantification   The Mass Spec Host Cell Protein (HCP) assay is applied for monitoring...

Oct 2. 2009 | Ejvind Mørtz |
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