Why is my protein’s N-terminal blocked (and what can I do about it)?

Question: I found that the N-terminal of my protein is blocked. How can I then analyze it by Edman degradation /...

Aug 31. 2017 | Ejvind Mørtz |
Protein molecular weight calculator

GPMAW lite – Protein Sequence Calculator (and much more)

When working with proteins you often need to make simple bioinformatics calculations on a specific protein sequence - and for...

Apr 10. 2017 | Thomas Kofoed |

Why you need accurate concentration determination of protein standards

Question: How do I quantify my protein standard? Is it true that it if it is done incorrectly it can affect...

Jan 18. 2017 | Thomas Kofoed |
Amino Acid analysis

4 things to remember about Amino Acid Analysis of proteins & peptides

Why perform Amino Acid Analysis? Amino acid analysis of proteins is a method to determine the absolute amounts of individual amino...

Sep 23. 2016 | Ejvind Mørtz |

What buffer to use for MALDI mass spectrometry analysis of a protein?

Question: What buffer should I use for MALDI Mass Spectrometry analysis of my protein sample? Answer: In general, MALDI-MS is more tolerant to contamination...

Apr 28. 2016 | Ejvind Mørtz |

What does the Mascot Score mean?

Question: I just got my protein identification report. What does the Mascot Score mean? Answer: The Protein Identification report contains a list of proteins that have...

Oct 9. 2015 | Ejvind Mørtz |

Optimize PVDF membrane electroblotting for N-terminal Edman sequencing

Question: How do I optimize electroblotting conditions onto PVDF membrane for N-terminal Edman sequencing? Our 12 kDa protein separates well on 1D...

Oct 13. 2013 | Ejvind Mørtz |
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