Protein calculator

GPMAW lite – Protein Molecular Weight Calculator (and much more)

Simple bioinformatics calculations on specific protein sequences   When working with proteins you often need to make simple bioinformatics calculations on a specific...

Apr 10. 2017 | Thomas Kofoed |

Why you need accurate concentration determination of protein standards

Question:   How do I quantify my protein standard?   Answer:   Have you ever questioned the quantitative data you get from Bradford, Lowry or BCA...

Jan 18. 2017 | Thomas Kofoed |
Amino Acid analysis

4 things to remember about Amino Acid Analysis of proteins & peptides

Why perform Amino Acid Analysis?   Amino acid analysis is a method to determine the absolute amounts of individual amino acids in...

Sep 23. 2016 | Ejvind Mørtz |
Preparation of sample for MALDI

What buffer to use for MALDI mass spectrometry analysis of a protein?

Question:   What buffer should I use for MALDI Mass Spectrometry analysis of my protein sample?   Answer:   In general, MALDI-MS is more tolerant to contamination...

Apr 28. 2016 | Ejvind Mørtz |

What does the Mascot Score mean?

Question:   I just got my protein identification report. What does the Mascot Score mean?   Answer:   The Protein Identification report contains a list of proteins that have...

Oct 9. 2015 | Ejvind Mørtz |

Why is my protein’s N-terminal blocked (and what can I do about it)?

Question:   I found that the N-terminal of my protein is blocked. How can I then analyze it by Edman degradation/sequencing?   Answer:   N-terminal sequencing...

Aug 31. 2015 | Ejvind Mørtz |

Optimize electroblotting on PVDF membrane for N-terminal Edman sequencing

Question:   How do I optimize electroblotting conditions onto PVDF membrane for N-terminal Edman sequencing? Our 12 kDa protein separates well on 1D...

Oct 13. 2013 | Ejvind Mørtz |

N- and C-terminal protein sequencing using MALDI ISD

Structural characterization of purified natural and recombinant proteins traditionally includes N-terminal Edman sequencing to confirm 5-15 amino acid residues from...

Aug 18. 2013 | Ejvind Mørtz |
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