Why you need accurate concentration determination of protein standards
Question: How do I quantify my protein standard? How do I quantify my protein standard? Is it true that it if it...
4 things to remember about Amino Acid Analysis of proteins & peptides
Why perform Amino Acid Analysis? Amino acid analysis of proteins is a method to determine the absolute amounts of individual amino...
How much protein should I load for protein identification by mass spec
Question: How should I prepare my samples for 2D electrophoresis? How should I prepare my samples for 2D electrophoresis, and how much...
What does the Mascot Score mean?
Question: What does the Mascot Score mean? I just got my protein identification report. What does the Mascot Score mean? Answer: The Protein Identification...
Preparing samples for N- and C-terminal sequencing by MALDI ISD
Question: How do I prepare samples for MALDI ISD analysis? I am interested in your N and C-terminal sequencing using MALDI ISD....
Optimize PVDF membrane electroblotting for N-terminal Edman sequencing
Question: How do I optimize electroblotting conditions onto a PVDF membrane? Our 12 kDa protein separates well on 1D SDS PAGE but...