Why you need accurate concentration determination of protein standards
Question: How do I quantify my protein standard? Answer: Have you ever questioned the quantitative data you get from Bradford, Lowry or BCA...
4 things to remember about Amino Acid Analysis of proteins & peptides
Why perform Amino Acid Analysis? Amino acid analysis is a method to determine the absolute amounts of individual amino acids in...
How much protein should I load for protein identification by mass spec
Question: How should I prepare my samples for 2D electrophoresis, and how much protein should I load to get protein ID...
What does the Mascot Score mean?
Question: I just got my protein identification report. What does the Mascot Score mean? Answer: The Protein Identification report contains a list of proteins that have...
Preparing samples for N- and C-terminal sequencing by MALDI ISD
Question: I am interested in your N and C-terminal sequencing using MALDI ISD. How do I prepare the sample for analysis? Answer: MALDI...
Optimize electroblotting on PVDF membrane for N-terminal Edman sequencing
Question: How do I optimize electroblotting conditions onto PVDF membrane for N-terminal Edman sequencing? Our 12 kDa protein separates well on 1D...
N- and C-terminal protein sequencing using MALDI ISD
Structural characterization of purified natural and recombinant proteins traditionally includes N-terminal Edman sequencing to confirm 5-15 amino acid residues from...